PMID- 10323459
OWN - NLM
STAT- MEDLINE
DCOM- 19990521
LR  - 20071114
IS  - 0004-3591 (Print)
IS  - 0004-3591 (Linking)
VI  - 42
IP  - 5
DP  - 1999 May
TI  - Immunization of mice with human 60-kd Ro peptides results in epitope spreading if
      the peptides are highly homologous between human and mouse.
PG  - 1017-24
AB  - OBJECTIVE: Immunization with peptide fragments of autoantigens may lead to an
      immune response at both the T and B cell level that is directed not only at the
      immunogen, but also at the autoantigen from which the peptide came. In addition, 
      a complex multicomponent particle may become the target of this expanded immune
      response. The purpose of this study was to determine the ability of several
      different peptides from 60-kd Ro to induce expansion of the immune response to
      the Ro/La RNP particle. METHODS: We immunized BALB/c mice with 3 different
      oligopeptides from human 60-kd Ro (or, SSA). RESULTS: Animals immunized with
      peptides either identical to or differing by only 1 amino acid developed
      autoimmunity to the entire Ro RNP particle. Animals immunized with a human
      peptide highly divergent from the corresponding mouse sequence developed an
      immune response to the immunogen only and showed little evidence of epitope
      spreading. Furthermore, these mice did not have antibodies that bound the poorly 
      conserved mouse homolog peptide, and the antibody response to this peptide did
      not include IgG1. CONCLUSION: These data indicate that B lymphocytes specific for
      the self-peptide that is homologous to the immunogen are a critical determinant
      for spreading of the immune response to other components of self.
FAU - Scofield, R H
AU  - Scofield RH
AD  - Oklahoma Medical Research Foundation, University of Oklahoma Health Sciences
      Center, Department of Veterans Affairs Medical Center, and WK Warren Medical
      Research Institute, Oklahoma City 73104, USA.
FAU - Kaufman, K M
AU  - Kaufman KM
FAU - Baber, U
AU  - Baber U
FAU - James, J A
AU  - James JA
FAU - Harley, J B
AU  - Harley JB
FAU - Kurien, B T
AU  - Kurien BT
LA  - eng
GR  - AI-24717/AI/NIAID NIH HHS/United States
GR  - AR-01844/AR/NIAMS NIH HHS/United States
GR  - AR-42460/AR/NIAMS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Arthritis Rheum
JT  - Arthritis and rheumatism
JID - 0370605
RN  - 0 (Autoantigens)
RN  - 0 (Epitopes)
RN  - 0 (RNA, Small Cytoplasmic)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (SS-A antigen)
RN  - 0 (TROVE2 protein, human)
SB  - AIM
SB  - IM
MH  - Animals
MH  - Antibody Formation
MH  - Autoantigens/*immunology
MH  - Autoimmunity/physiology
MH  - Epitopes
MH  - Humans
MH  - Immunization
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *RNA, Small Cytoplasmic
MH  - Ribonucleoproteins/*immunology
MH  - Sequence Homology, Amino Acid
EDAT- 1999/05/14 00:00
MHDA- 1999/05/14 00:01
CRDT- 1999/05/14 00:00
PHST- 1999/05/14 00:00 [pubmed]
PHST- 1999/05/14 00:01 [medline]
PHST- 1999/05/14 00:00 [entrez]
AID - 10.1002/1529-0131(199905)42:5<1017::AID-ANR22>3.0.CO;2-7 [doi]
PST - ppublish
SO  - Arthritis Rheum. 1999 May;42(5):1017-24. doi:
      10.1002/1529-0131(199905)42:5<1017::AID-ANR22>3.0.CO;2-7.