PMID- 10322635
OWN - NLM
STAT- MEDLINE
DCOM- 19990701
LR  - 20061115
IS  - 0192-253X (Print)
IS  - 0192-253X (Linking)
VI  - 24
IP  - 3-4
DP  - 1999
TI  - Screen for genes regulated during early kidney morphogenesis.
PG  - 273-83
AB  - Kidney development starts with the reciprocal induction of mesenchymal and
      ureteric bud cells which leads to condensation, epithelialization, and nephron
      formation in the mesenchyme. To identify changes in gene expression during these 
      processes, we compared differential display polymerase chain reaction (PCR)
      profiles of uninduced and induced mesenchymal cells. In vitro kidney development 
      in the form of the transfilter organ culture system was used to generate
      homogeneous cell populations for this type of comparison. Here we describe the
      isolation of known and novel genetags from this screening. Among the known genes 
      the ufo receptor tyrosine kinase, sFRP2, and the groucho related gene (grg) were 
      verified as being upregulated upon induction. With four of eight novel genes
      tested, Northern blot analysis proved to be sensitive enough to confirm
      differential expression. To improve sensitivity and gain additional spatial
      information, in situ hybridization was performed. Expression analysis of two
      differential display PCR products, designated C0-5 and M2-4, demonstrated the
      cell-specific and dynamic expression of these novel genetags in the developing
      kidney and other tissues. C0-5 transcripts were expressed in the ureteric bud,
      S-shaped bodies, and in the collecting system. Signals for M2-4, a gene not
      detectable by Northern blot analysis, were only found in condensing mesenchymal
      cells and early differentiation stages, but not in the collecting ducts. The
      large fraction of novel genetags from the present screening that have not yet
      been analyzed provides a rich resource to clone genetic networks regulating early
      nephrogenesis.
FAU - Leimeister, C
AU  - Leimeister C
AD  - Department of Physiological Chemistry I, Theodor-Boveri-Institute (Biocenter),
      University of Wuerzburg, Germany.
FAU - Bach, A
AU  - Bach A
FAU - Woolf, A S
AU  - Woolf AS
FAU - Gessler, M
AU  - Gessler M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Genet
JT  - Developmental genetics
JID - 7909963
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - DNA Primers/genetics
MH  - Expressed Sequence Tags
MH  - *Gene Expression Regulation, Developmental
MH  - In Situ Hybridization
MH  - Kidney/*embryology/metabolism
MH  - Mesoderm/metabolism
MH  - Mice
MH  - Polymerase Chain Reaction/methods
MH  - RNA, Messenger/genetics/metabolism
EDAT- 1999/05/14 02:02
MHDA- 2000/06/20 09:00
CRDT- 1999/05/14 02:02
PHST- 1999/05/14 02:02 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/05/14 02:02 [entrez]
AID - 10.1002/(SICI)1520-6408(1999)24:3/4<273::AID-DVG10>3.0.CO;2-3 [pii]
AID - 10.1002/(SICI)1520-6408(1999)24:3/4<273::AID-DVG10>3.0.CO;2-3 [doi]
PST - ppublish
SO  - Dev Genet. 1999;24(3-4):273-83. doi:
      10.1002/(SICI)1520-6408(1999)24:3/4<273::AID-DVG10>3.0.CO;2-3.