PMID- 10322631
OWN - NLM
STAT- MEDLINE
DCOM- 19990701
LR  - 20101118
IS  - 0192-253X (Print)
IS  - 0192-253X (Linking)
VI  - 24
IP  - 3-4
DP  - 1999
TI  - Metanephric rat-mouse chimeras to study cell lineage of the nephron.
PG  - 230-40
AB  - The nephron is derived from the ureteric bud and metanephric mesenchyme and
      develops into a complex epithelial structure with a wide variety of phenotypes
      along its length. This segmental variation in expression of molecules provides an
      approach to understand the lineage of unique segments. The present study
      evaluated the expression of four relatively well-localized molecules--renin,
      Tamm-Horsfall protein (THP), oxytocin receptor (OTR), and the vasopressin type 2 
      receptor (V2R)--in cultured mouse-rat chimeric metanephric kidneys using reverse 
      transcription-polymerase chain reaction (RT-PCR). Chimeric kidneys were formed by
      1) separating the ureteric bud (U) from the metanephric mesenchyme (M) of mouse
      (m) at E11 and rat (r) at E13 days of gestation and 2) recombining the ureteric
      bud of one species with the metanephric mesenchyme of the other species (i.e.,
      UrMm and UmM(r), followed by filter culture until differentiated.
      Species-specific restriction enzymes for all four genes were chosen to digest the
      PCR product from either rat or mouse. RT-PCR was performed for each mRNA species 
      and the products digested. The V2R product from the UrMm chimera was cleaved by a
      restriction enzyme known to digest only rat product, suggesting the PCR product
      was produced predominantly by cells derived from the ureteric bud. The renin,
      OTR, and THP products from both chimeras were cleaved equally well by
      species-specific restriction enzymes, suggesting the products were made by cells 
      originating from both the ureteric bud and the metanephric mesenchyme. These
      studies demonstrate that the cultured chimeric metanephric model is useful to
      study segment lineage. The results suggest that the lineage of at least certain
      portions of the nephron is heterogenous.
FAU - Arend, L J
AU  - Arend LJ
AD  - Department of Pathology, University of Michigan, Ann Arbor, USA. larend@umich.edu
FAU - Smart, A
AU  - Smart A
FAU - Briggs, J P
AU  - Briggs JP
LA  - eng
GR  - DK09325/DK/NIDDK NIH HHS/United States
GR  - DK37448/DK/NIDDK NIH HHS/United States
GR  - DK39255/DK/NIDDK NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Dev Genet
JT  - Developmental genetics
JID - 7909963
RN  - 0 (DNA Primers)
RN  - 0 (Mucoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Oxytocin)
RN  - 0 (Receptors, Vasopressin)
RN  - 0 (Umod protein, mouse)
RN  - 0 (Umod protein, rat)
RN  - 0 (Uromodulin)
RN  - EC 3.4.23.15 (Renin)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - *Chimera/genetics
MH  - DNA Primers/genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mucoproteins/genetics
MH  - Nephrons/*cytology/*embryology/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Receptors, Oxytocin/genetics
MH  - Receptors, Vasopressin/genetics
MH  - Renin/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Nucleic Acid
MH  - Species Specificity
MH  - Uromodulin
EDAT- 1999/05/14 02:02
MHDA- 2000/06/20 09:00
CRDT- 1999/05/14 02:02
PHST- 1999/05/14 02:02 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/05/14 02:02 [entrez]
AID - 10.1002/(SICI)1520-6408(1999)24:3/4<230::AID-DVG6>3.0.CO;2-Y [pii]
AID - 10.1002/(SICI)1520-6408(1999)24:3/4<230::AID-DVG6>3.0.CO;2-Y [doi]
PST - ppublish
SO  - Dev Genet. 1999;24(3-4):230-40. doi:
      10.1002/(SICI)1520-6408(1999)24:3/4<230::AID-DVG6>3.0.CO;2-Y.