PMID- 10321728
OWN - NLM
STAT- MEDLINE
DCOM- 19990603
LR  - 20190605
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 12
DP  - 1999 Mar 25
TI  - HOS, a human homolog of Slimb, forms an SCF complex with Skp1 and Cullin1 and
      targets the phosphorylation-dependent degradation of IkappaB and beta-catenin.
PG  - 2039-46
AB  - SCF E3 ubiquitin ligases mediate ubiquitination and proteasome-dependent
      degradation of phosphorylated substrates. We identified a human F-box/WD40
      repeats protein (HOS), which is homologous to Slimb/h betaTrCP. Being a part of
      SCF complex with Skp1 and Cullin1, HOS specifically interacted with the
      phosphorylated IkappaB and beta-catenin, targeting these proteins for
      proteasome-dependent degradation in vivo. This targeting required Cullin1 as
      expression of a mutant Cullin1 abrogated the degradation of IkappaB and of
      beta-catenin. Mutant HOS which lacks the F-box blocked TNF alpha-induced
      degradation of IkappaB as well as GSK3beta-mediated degradation of beta-catenin. 
      This mutant also inhibited NF-kappaB transactivation and increased the
      beta-catenin-dependent transcription activity of Tcf. These results demonstrate
      that SCF(HOS) E3 ubiquitin ligase regulate both NF-kappaB and beta-catenin
      signaling pathways.
FAU - Fuchs, S Y
AU  - Fuchs SY
AD  - Derald H Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York, NY
      10029-6574, USA.
FAU - Chen, A
AU  - Chen A
FAU - Xiong, Y
AU  - Xiong Y
FAU - Pan, Z Q
AU  - Pan ZQ
FAU - Ronai, Z
AU  - Ronai Z
LA  - eng
SI  - GENBANK/AB014596
GR  - CA 78419/CA/NCI NIH HHS/United States
GR  - GM 55059/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (CTNNB1 protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (FBXW11 protein, human)
RN  - 0 (Insect Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Trans-Activators)
RN  - 0 (beta Catenin)
RN  - 0 (beta-Transducin Repeat-Containing Proteins)
RN  - 0 (slmb protein, Drosophila)
RN  - EC 2.3.2.27 (SKP Cullin F-Box Protein Ligases)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 6.- (Ligases)
RN  - EC 6.3.2.- (Peptide Synthases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cell Cycle Proteins/genetics
MH  - Cytoskeletal Proteins/*metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - *Drosophila Proteins
MH  - Humans
MH  - Insect Proteins/genetics
MH  - Ligases/*metabolism
MH  - Molecular Sequence Data
MH  - NF-kappa B/*antagonists & inhibitors/metabolism
MH  - Peptide Synthases/*metabolism
MH  - Phosphorylation
MH  - Protein Binding
MH  - Protein Processing, Post-Translational
MH  - SKP Cullin F-Box Protein Ligases
MH  - Sequence Homology, Amino Acid
MH  - *Trans-Activators
MH  - Transcriptional Activation
MH  - Ubiquitin-Protein Ligases
MH  - beta Catenin
MH  - *beta-Transducin Repeat-Containing Proteins
EDAT- 1999/05/13 00:00
MHDA- 1999/05/13 00:01
CRDT- 1999/05/13 00:00
PHST- 1999/05/13 00:00 [pubmed]
PHST- 1999/05/13 00:01 [medline]
PHST- 1999/05/13 00:00 [entrez]
AID - 10.1038/sj.onc.1202760 [doi]
PST - ppublish
SO  - Oncogene. 1999 Mar 25;18(12):2039-46. doi: 10.1038/sj.onc.1202760.