PMID- 10321247
OWN - NLM
STAT- MEDLINE
DCOM- 19990525
LR  - 20071114
IS  - 1097-6256 (Print)
IS  - 1097-6256 (Linking)
VI  - 2
IP  - 5
DP  - 1999 May
TI  - The septin CDCrel-1 binds syntaxin and inhibits exocytosis.
PG  - 434-9
AB  - Septins are GTPases required for the completion of cytokinesis in diverse
      organisms, yet their roles in cytokinesis or other cellular processes remain
      unknown. Here we describe studies of a newly identified septin, CDCrel-1, which
      is predominantly expressed in the nervous system. This protein was associated
      with membrane fractions, and a significant fraction of the protein copurified and
      coprecipitated with synaptic vesicles. In detergent extracts, CDCrel-1 and
      another septin, Nedd5, immunoprecipitated with the SNARE protein syntaxin by
      directly binding to syntaxin via the SNARE interaction domain. Transfection of
      HIT-T15 cells with wild-type CDCrel-1 inhibited secretion, whereas GTPase
      dominant-negative mutants enhanced secretion. These data suggest that septins may
      regulate vesicle dynamics through interactions with syntaxin.
FAU - Beites, C L
AU  - Beites CL
AD  - Program in Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada.
FAU - Xie, H
AU  - Xie H
FAU - Bowser, R
AU  - Bowser R
FAU - Trimble, W S
AU  - Trimble WS
LA  - eng
GR  - AG13208/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Nat Neurosci
JT  - Nature neuroscience
JID - 9809671
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Qa-SNARE Proteins)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
SB  - IM
MH  - Animals
MH  - Cell Membrane/metabolism
MH  - Cytoskeletal Proteins/metabolism
MH  - Exocytosis/*physiology
MH  - GTP Phosphohydrolases/*metabolism
MH  - Gene Library
MH  - Humans
MH  - Hybridomas
MH  - Immunohistochemistry
MH  - Membrane Proteins/*metabolism
MH  - Nerve Tissue Proteins/*metabolism
MH  - PC12 Cells
MH  - Protein Binding
MH  - Qa-SNARE Proteins
MH  - Rats
MH  - Transfection
EDAT- 1999/05/13 02:04
MHDA- 2001/03/23 10:01
CRDT- 1999/05/13 02:04
PHST- 1999/05/13 02:04 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/05/13 02:04 [entrez]
AID - 10.1038/8100 [doi]
PST - ppublish
SO  - Nat Neurosci. 1999 May;2(5):434-9. doi: 10.1038/8100.