PMID- 10321245
OWN - NLM
STAT- MEDLINE
DCOM- 19990525
LR  - 20181201
IS  - 1097-6256 (Print)
IS  - 1097-6256 (Linking)
VI  - 2
IP  - 5
DP  - 1999 May
TI  - Inhalational anesthetics activate two-pore-domain background K+ channels.
PG  - 422-6
AB  - Volatile anesthetics produce safe, reversible unconsciousness, amnesia and
      analgesia via hyperpolarization of mammalian neurons. In molluscan pacemaker
      neurons, they activate an inhibitory synaptic K+ current (IKAn), proposed to be
      important in general anesthesia. Here we show that TASK and TREK-1, two recently 
      cloned mammalian two-P-domain K+ channels similar to IKAn in biophysical
      properties, are activated by volatile general anesthetics. Chloroform, diethyl
      ether, halothane and isoflurane activated TREK-1, whereas only halothane and
      isoflurane activated TASK. Carboxy (C)-terminal regions were critical for
      anesthetic activation in both channels. Thus both TREK-1 and TASK are possibly
      important target sites for these agents.
FAU - Patel, A J
AU  - Patel AJ
AD  - Institut de Pharmacologie Moleculaire et Cellulaire-CNRS-UPR 411, Valbonne,
      France.
FAU - Honore, E
AU  - Honore E
FAU - Lesage, F
AU  - Lesage F
FAU - Fink, M
AU  - Fink M
FAU - Romey, G
AU  - Romey G
FAU - Lazdunski, M
AU  - Lazdunski M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Nat Neurosci
JT  - Nature neuroscience
JID - 9809671
RN  - 0 (Anesthetics, Inhalation)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Potassium Channels)
RN  - 0 (Potassium Channels, Tandem Pore Domain)
RN  - 0 (potassium channel protein TREK-1)
RN  - 1HQ3YCN4GS (potassium channel subfamily K member 3)
SB  - IM
MH  - Amino Acid Sequence
MH  - Anesthetics, Inhalation/*pharmacology
MH  - Animals
MH  - COS Cells
MH  - Lymnaea
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins
MH  - Neurons/*drug effects
MH  - Patch-Clamp Techniques
MH  - Porosity
MH  - Potassium Channels/*drug effects
MH  - *Potassium Channels, Tandem Pore Domain
MH  - *Protein Structure, Tertiary
MH  - Sequence Homology, Amino Acid
EDAT- 1999/05/13 02:04
MHDA- 2001/03/23 10:01
CRDT- 1999/05/13 02:04
PHST- 1999/05/13 02:04 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/05/13 02:04 [entrez]
AID - 10.1038/8084 [doi]
PST - ppublish
SO  - Nat Neurosci. 1999 May;2(5):422-6. doi: 10.1038/8084.