PMID- 10320326
OWN - NLM
STAT- MEDLINE
DCOM- 19990607
LR  - 20131121
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 38
IP  - 19
DP  - 1999 May 11
TI  - The structure of retinal dehydrogenase type II at 2.7 A resolution: implications 
      for retinal specificity.
PG  - 6003-11
AB  - Retinoic acid, a hormonally active form of vitamin A, is produced in vivo in a
      two step process: retinol is oxidized to retinal and retinal is oxidized to
      retinoic acid. Retinal dehydrogenase type II (RalDH2) catalyzes this last step in
      the production of retinoic acid in the early embryo, possibly producing this
      putative morphogen to initiate pattern formation. The enzyme is also found in the
      adult animal, where it is expressed in the testis, lung, and brain among other
      tissues. The crystal structure of retinal dehydrogenase type II cocrystallized
      with nicotinamide adenine dinucleotide (NAD) has been determined at 2.7 A
      resolution. The structure was solved by molecular replacement using the crystal
      structure of a mitochondrial aldehyde dehydrogenase (ALDH2) as a model. Unlike
      what has been described for the structures of two aldehyde dehydrogenases
      involved in the metabolism of acetaldehyde, the substrate access channel is not a
      preformed cavity into which acetaldehyde can readily diffuse. Retinal
      dehydrogenase appears to utilize a disordered loop in the substrate access
      channel to discriminate between retinaldehyde and short-chain aldehydes.
FAU - Lamb, A L
AU  - Lamb AL
AD  - Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, 
      Tennessee 37232, USA.
FAU - Newcomer, M E
AU  - Newcomer ME
LA  - eng
SI  - PDB/1B19
GR  - GM0820/GM/NIGMS NIH HHS/United States
GR  - GM55420/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Aldehydes)
RN  - 0U46U6E8UK (NAD)
RN  - EC 1.2.- (Aldehyde Oxidoreductases)
RN  - EC 1.2.1.36 (Retinal Dehydrogenase)
SB  - IM
MH  - Aldehyde Oxidoreductases/*chemistry
MH  - Aldehydes/chemistry
MH  - Amino Acid Sequence
MH  - Animals
MH  - Catalytic Domain
MH  - Crystallography, X-Ray
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - NAD/*chemistry
MH  - Protein Conformation
MH  - Retinal Dehydrogenase
MH  - Sequence Homology, Amino Acid
EDAT- 1999/05/13 00:00
MHDA- 1999/05/13 00:01
CRDT- 1999/05/13 00:00
PHST- 1999/05/13 00:00 [pubmed]
PHST- 1999/05/13 00:01 [medline]
PHST- 1999/05/13 00:00 [entrez]
AID - 10.1021/bi9900471 [doi]
AID - bi9900471 [pii]
PST - ppublish
SO  - Biochemistry. 1999 May 11;38(19):6003-11. doi: 10.1021/bi9900471.