PMID- 10318869
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 20
DP  - 1999 May 14
TI  - MAPKAP kinase 2 phosphorylates serum response factor in vitro and in vivo.
PG  - 14434-43
AB  - Several growth factor- and calcium-regulated kinases such as pp90(rsk) or CaM
      kinase IV can phosphorylate the transcription factor serum response factor (SRF) 
      at serine 103 (Ser-103). However, it is unknown whether stress-regulated kinases 
      can also phosphorylate SRF. We show that treatment of cells with anisomycin,
      arsenite, sodium fluoride, or tetrafluoroaluminate induces phosphorylation of SRF
      at Ser-103 in both HeLa and NIH3T3 cells. This phosphorylation is dependent on
      the kinase p38/SAPK2 and correlates with the activation of MAPKAP kinase 2 (MK2).
      MK2 phosphorylates SRF in vitro at Ser-103 with similar efficiency as the small
      heat shock protein Hsp25 and significantly better than CREB. Comparison of wild
      type murine fibroblasts with those derived from MK2-deficient mice (Mk(-/-))
      reveals MK2 as the major SRF kinase induced by arsenite. These results
      demonstrate that SRF is targeted by several signal transduction pathways within
      cells and establishes SRF as a nuclear target for MAPKAP kinase 2.
FAU - Heidenreich, O
AU  - Heidenreich O
AD  - Institut fur Zellbiologie, Abteilung Molekularbiologie, Universitat Tubingen,
      D-72076 Tubingen, Germany.
FAU - Neininger, A
AU  - Neininger A
FAU - Schratt, G
AU  - Schratt G
FAU - Zinck, R
AU  - Zinck R
FAU - Cahill, M A
AU  - Cahill MA
FAU - Engel, K
AU  - Engel K
FAU - Kotlyarov, A
AU  - Kotlyarov A
FAU - Kraft, R
AU  - Kraft R
FAU - Kostka, S
AU  - Kostka S
FAU - Gaestel, M
AU  - Gaestel M
FAU - Nordheim, A
AU  - Nordheim A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Arsenites)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Serum Response Factor)
RN  - 0 (Teratogens)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.1.- (MAP-kinase-activated kinase 2)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - N5509X556J (arsenite)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Arsenites/pharmacology
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Fibroblasts/drug effects/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Mice
MH  - *Mitogen-Activated Protein Kinases
MH  - Nuclear Proteins/*metabolism
MH  - Phosphorylation
MH  - Protein-Serine-Threonine Kinases/*metabolism
MH  - Serine/metabolism
MH  - Serum Response Factor
MH  - Signal Transduction/drug effects
MH  - Teratogens/pharmacology
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 1999/05/13 00:00
MHDA- 1999/05/13 00:01
CRDT- 1999/05/13 00:00
PHST- 1999/05/13 00:00 [pubmed]
PHST- 1999/05/13 00:01 [medline]
PHST- 1999/05/13 00:00 [entrez]
AID - 10.1074/jbc.274.20.14434 [doi]
AID - S0021-9258(19)73321-3 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 May 14;274(20):14434-43. doi: 10.1074/jbc.274.20.14434.