PMID- 10318867
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 20
DP  - 1999 May 14
TI  - Myb-interacting protein, ATBF1, represses transcriptional activity of Myb
      oncoprotein.
PG  - 14422-8
AB  - Using the yeast two-hybrid system, the transcription factor ATBF1 was identified 
      as v-Myb- and c-Myb-binding protein. Deletion mutagenesis revealed amino acids
      2484-2520 in human ATBF1 and 279-300 in v-Myb as regions required for in vitro
      binding of both proteins. Further experiments identified leucines Leu325 and
      Leu332 of the Myb leucine zipper motif as additional amino acid residues
      important for efficient ATBF1-Myb interaction in vitro. In co-transfection
      experiments, the full-length ATBF1 was found to form in vivo complexes with v-Myb
      and inhibit v-Myb transcriptional activity. Both ATBF1 2484-2520 and Myb 279-300 
      regions were required for the inhibitory effect. Finally, the chicken ATBF1 was
      identified, showing high degree of amino acid sequence homology with human and
      murine proteins. Our data reveal Myb proteins as the first ATBF1 partners
      detected so far and identify amino acids 279-300 in v-Myb as a novel
      protein-protein interaction interface through which Myb transcriptional activity 
      can be regulated.
FAU - Kaspar, P
AU  - Kaspar P
AD  - Institute of Molecular Genetics, Academy of Sciences of the Czech Republic,
      Flemingovo 2, 166 37 Prague 6, Czech Republic.
FAU - Dvorakova, M
AU  - Dvorakova M
FAU - Kralova, J
AU  - Kralova J
FAU - Pajer, P
AU  - Pajer P
FAU - Kozmik, Z
AU  - Kozmik Z
FAU - Dvorak, M
AU  - Dvorak M
LA  - eng
SI  - GENBANK/AF104331
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Oncogene Proteins v-myb)
RN  - 0 (Retroviridae Proteins, Oncogenic)
RN  - 143258-00-8 (ZFHX3 protein, human)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Conserved Sequence
MH  - DNA-Binding Proteins/*metabolism
MH  - Homeodomain Proteins/*metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/metabolism
MH  - Oncogene Proteins v-myb
MH  - Protein-Tyrosine Kinases/*metabolism
MH  - Retroviridae Proteins, Oncogenic/*metabolism
MH  - Structure-Activity Relationship
MH  - *Transcription, Genetic
MH  - *Zinc Fingers
EDAT- 1999/05/13 00:00
MHDA- 1999/05/13 00:01
CRDT- 1999/05/13 00:00
PHST- 1999/05/13 00:00 [pubmed]
PHST- 1999/05/13 00:01 [medline]
PHST- 1999/05/13 00:00 [entrez]
AID - 10.1074/jbc.274.20.14422 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 May 14;274(20):14422-8. doi: 10.1074/jbc.274.20.14422.