PMID- 10318856
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 20
DP  - 1999 May 14
TI  - The role of human TFIIB in transcription start site selection in vitro and in
      vivo.
PG  - 14337-43
AB  - The general transcription factor TFIIB plays a crucial role in selecting the
      transcription initiation site in yeast. We have analyzed the human homologs of
      TFIIB mutants that have previously been shown to affect transcription start site 
      selection in the yeast Saccharomyces cerevisiae. Despite the distinct mechanisms 
      of transcription start site selection observed in S. cerevisiae and humans, the
      role of TFIIB in this process is similar. However, unlike their yeast
      counterparts, the human mutants do not show a severe defect in supporting either 
      basal transcription or transcription stimulated by an acidic activator in vitro. 
      Transient transfection analysis revealed that, in addition to a role in
      transcription start site selection, human TFIIB residue Arg-66 performs a
      critical function in vivo that is bypassed in vitro. Furthermore, although
      correct transcription start site selection is dependent upon an arginine residue 
      at position 66 in human TFIIB, innate function in vivo is determined by the
      charge of the residue alone. Our observations raise questions as to the
      evolutionary conservation of TFIIB and uncover an additional function for TFIIB
      that is required in vivo but can be bypassed in vitro.
FAU - Hawkes, N A
AU  - Hawkes NA
AD  - Division of Gene Expression, Department of Biochemistry, Wellcome Trust Building,
      University of Dundee, Dundee DD1 5EH, United Kingdom.
FAU - Roberts, S G
AU  - Roberts SG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adenovirus E4 Proteins)
RN  - 0 (Transcription Factor TFIIB)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Adenovirus E4 Proteins/genetics
MH  - Amino Acid Sequence
MH  - Cell Line
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Promoter Regions, Genetic
MH  - Saccharomyces cerevisiae
MH  - Transcription Factor TFIIB
MH  - Transcription Factors/*metabolism
MH  - *Transcription, Genetic
EDAT- 1999/05/13 00:00
MHDA- 1999/05/13 00:01
CRDT- 1999/05/13 00:00
PHST- 1999/05/13 00:00 [pubmed]
PHST- 1999/05/13 00:01 [medline]
PHST- 1999/05/13 00:00 [entrez]
AID - 10.1074/jbc.274.20.14337 [doi]
AID - S0021-9258(19)73308-0 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 May 14;274(20):14337-43. doi: 10.1074/jbc.274.20.14337.