PMID- 10318855
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 20
DP  - 1999 May 14
TI  - Heterodimeric interactions between chicken ovalbumin upstream
      promoter-transcription factor family members ARP1 and ear2.
PG  - 14331-6
AB  - Members of the chicken ovalbumin upstream promoter-transcription factor (COUP-TF)
      subfamily of orphan nuclear receptors, which minimally includes COUP-TFI and
      ARP1, are highly expressed in brain and are generally considered to be
      constitutive repressors of transcription. We have used a yeast two-hybrid system 
      to isolate proteins expressed in brain that interact with ARP1. One of the
      proteins isolated in this screen was Ear2, another orphan receptor that has been 
      suggested to be a member of the COUP-TF subfamily. Here we demonstrate that ARP1 
      and Ear2 form heterodimers in solution and on directly repeated response elements
      with high efficiency and a specificity differing from that of homodimeric
      complexes composed of either receptor. ARP1 and Ear2 were observed to interact in
      mammalian cells, and the tissue distribution of Ear2 transcripts was found to
      overlap precisely with the expression pattern of ARP1 in several mouse tissues
      and embryonal carcinoma cell lines. Heterodimeric interactions between ARP1 and
      Ear2 may define a distinct pathway of orphan receptor signaling.
FAU - Avram, D
AU  - Avram D
AD  - Laboratory of Molecular Pharmacology, College of Pharmacy, Oregon State
      University, Corvallis, Oregon 97331-3507, USA.
FAU - Ishmael, J E
AU  - Ishmael JE
FAU - Nevrivy, D J
AU  - Nevrivy DJ
FAU - Peterson, V J
AU  - Peterson VJ
FAU - Lee, S H
AU  - Lee SH
FAU - Dowell, P
AU  - Dowell P
FAU - Leid, M
AU  - Leid M
LA  - eng
GR  - P30 ES000210/ES/NIEHS NIH HHS/United States
GR  - P30 ES000210-35/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Avian Proteins)
RN  - 0 (COUP Transcription Factor I)
RN  - 0 (COUP Transcription Factor II)
RN  - 0 (COUP Transcription Factors)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (EAR2 protein, Gallus gallus)
RN  - 0 (NR2F1 protein, human)
RN  - 0 (NR2F2 protein, human)
RN  - 0 (Nr2f6 protein, mouse)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Receptors, Steroid)
RN  - 0 (Transcription Factors)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - *Avian Proteins
MH  - COUP Transcription Factor I
MH  - COUP Transcription Factor II
MH  - COUP Transcription Factors
MH  - Cell Line
MH  - Chickens
MH  - DNA-Binding Proteins/*metabolism
MH  - Dimerization
MH  - Humans
MH  - Ovalbumin/genetics/metabolism
MH  - *Promoter Regions, Genetic
MH  - Protein Binding
MH  - *Receptors, Cytoplasmic and Nuclear
MH  - Receptors, Glucocorticoid/*metabolism
MH  - *Receptors, Steroid
MH  - Transcription Factors/*metabolism
PMC - PMC2823254
MID - NIHMS173647
EDAT- 1999/05/13 00:00
MHDA- 1999/05/13 00:01
CRDT- 1999/05/13 00:00
PHST- 1999/05/13 00:00 [pubmed]
PHST- 1999/05/13 00:01 [medline]
PHST- 1999/05/13 00:00 [entrez]
AID - 10.1074/jbc.274.20.14331 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 May 14;274(20):14331-6. doi: 10.1074/jbc.274.20.14331.