PMID- 10318838
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 20
DP  - 1999 May 14
TI  - GTP-dependent binding of ADP-ribosylation factor to coatomer in close proximity
      to the binding site for dilysine retrieval motifs and p23.
PG  - 14198-203
AB  - A site-directed photocross-linking approach was employed to determine components 
      that act downstream of ADP-ribosylation factor (ARF). To this end, a photolabile 
      phenylalanine analog was incorporated at various positions of the putative
      effector region of the ARF molecule. Depending on the position of incorporation, 
      we find specific and GTP-dependent interactions of ARF with two subunits of the
      coatomer complex, beta-COP and gamma-COP, as well as an interaction with a
      cytosolic protein (approximately 185 kDa). In addition, we observe homodimer
      formation of ARF molecules at the Golgi membrane. These data suggest that the
      binding site of ARF to coatomer is at the interface of its beta- and
      gamma-subunits, and this is in close proximity to the second site of interaction 
      of coatomer with the Golgi membrane, the binding site within gamma-COP for
      cytosolic dibasic/diphenylalanine motifs.
FAU - Zhao, L
AU  - Zhao L
AD  - Biochemie-Zentrum Heidelberg, University of Heidelberg, Im Neuenheimer Feld 328, 
      69120 Heidelberg, Germany.
FAU - Helms, J B
AU  - Helms JB
FAU - Brunner, J
AU  - Brunner J
FAU - Wieland, F T
AU  - Wieland FT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Coatomer Protein)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Membrane Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (ADP-Ribosylation Factors)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - ADP-Ribosylation Factors
MH  - Animals
MH  - Binding Sites
MH  - CHO Cells
MH  - Carrier Proteins/*metabolism
MH  - Cattle
MH  - Coatomer Protein
MH  - Cricetinae
MH  - Cross-Linking Reagents/metabolism
MH  - Crystallography, X-Ray
MH  - Dimerization
MH  - GTP-Binding Proteins/*metabolism
MH  - Golgi Apparatus/metabolism
MH  - Guanosine Triphosphate/*metabolism
MH  - Lysine/*metabolism
MH  - Macromolecular Substances
MH  - Membrane Proteins/*metabolism
MH  - Microtubule-Associated Proteins/metabolism
MH  - Molecular Weight
MH  - Photochemistry
MH  - Protein Conformation
MH  - Protein Structure, Secondary
MH  - Rabbits
EDAT- 1999/05/13 00:00
MHDA- 1999/05/13 00:01
CRDT- 1999/05/13 00:00
PHST- 1999/05/13 00:00 [pubmed]
PHST- 1999/05/13 00:01 [medline]
PHST- 1999/05/13 00:00 [entrez]
AID - 10.1074/jbc.274.20.14198 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 May 14;274(20):14198-203. doi: 10.1074/jbc.274.20.14198.