PMID- 10318830
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 20
DP  - 1999 May 14
TI  - NMR solution structure of complement-like repeat CR8 from the low density
      lipoprotein receptor-related protein.
PG  - 14130-6
AB  - The low density lipoprotein receptor-related protein is a member of the low
      density lipoprotein receptor family and contains clusters of cysteine-rich
      complement-like repeats of about 42 residues that are present in all members of
      this family of receptors. These clusters are thought to be the principal binding 
      sites for protein ligands. We have expressed one complement-like repeat, CR8,
      from the cluster in lipoprotein receptor-related protein that binds certain
      proteinase inhibitor-proteinase complexes and used three-dimensional NMR on the
      13C/15N-labeled protein to determine the structure in solution of the
      calcium-bound form. The structure is very similar in overall fold to repeat 5
      from the low density lipoprotein receptor (LB5), with backbone root mean square
      deviation of 1.5 A. The calcium-binding site also appears to be homologous, with 
      four carboxyl and two backbone carbonyl ligands. However, differences in primary 
      structure are such that equivalent surfaces that might represent the binding
      interfaces are very different from one another, indicating that different domains
      will have very different ligand specificities.
FAU - Huang, W
AU  - Huang W
AD  - Department of Biochemistry and Molecular Biology, College of Medicine, University
      of Illinois at Chicago, Chicago, Illinois 60612-4316, USA.
FAU - Dolmer, K
AU  - Dolmer K
FAU - Gettins, P G
AU  - Gettins PG
LA  - eng
SI  - PDB/1CR8
GR  - GM54414/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Ligands)
RN  - 0 (Low Density Lipoprotein Receptor-Related Protein-1)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, LDL)
RN  - 0 (Solutions)
RN  - 0 (alpha-Macroglobulins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Calcium/metabolism
MH  - Cloning, Molecular
MH  - Ligands
MH  - Low Density Lipoprotein Receptor-Related Protein-1
MH  - Magnetic Resonance Spectroscopy
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Peptide Fragments/chemistry
MH  - Protein Folding
MH  - Receptors, Immunologic/*chemistry
MH  - Receptors, LDL/*chemistry
MH  - Sequence Alignment
MH  - Solutions
MH  - alpha-Macroglobulins/*chemistry
EDAT- 1999/05/13 00:00
MHDA- 1999/05/13 00:01
CRDT- 1999/05/13 00:00
PHST- 1999/05/13 00:00 [pubmed]
PHST- 1999/05/13 00:01 [medline]
PHST- 1999/05/13 00:00 [entrez]
AID - 10.1074/jbc.274.20.14130 [doi]
AID - S0021-9258(19)73282-7 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 May 14;274(20):14130-6. doi: 10.1074/jbc.274.20.14130.