PMID- 10318760
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20061115
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 112 ( Pt 11)
DP  - 1999 Jun
TI  - The putative nuclear receptor mediator TIF1alpha is tightly associated with
      euchromatin.
PG  - 1671-83
AB  - Ligand-dependent transcriptional regulation by nuclear receptors is believed to
      be mediated by intermediary factors (TIFs) acting on remodelling of the chromatin
      structure and/or the activity of the transcriptional machinery. The putative
      transcriptional mediator TIF1alpha is a nuclear protein kinase that has been
      identified via its interaction with liganded nuclear receptors, including
      retinoic acid (RAR), retinoid X (RXR) and estrogen (ER) receptors. Here, we
      demonstrate that TIF1alpha is a non-histone chromosomal protein tightly
      associated with highly accessible euchromatic regions of the genome.
      Immunofluorescence confocal microscopy reveals that TIF1alpha exhibits a finely
      granular distribution in euchromatin of interphase nuclei, while it is mostly
      excluded from condensed chromatin and metaphase chromosomes. Immunoelectron
      microscopy shows that, in contrast to the heterochromatin protein HP1alpha, most 
      of TIF1alpha is associated with euchromatin, where it is preferentially localised
      on regions known to be sites for RNA polymerase II (perichromatin fibrils and
      borders between euchromatin and heterochromatin). Early mouse embryos as well as 
      embryonal carcinoma (EC) and embryonic stem (ES) cells express high levels of
      TIF1alpha. These levels dramatically decrease during organogenesis and upon
      differentiation of P19 EC cells, indicating that TIF1alpha is preferentially
      expressed in undifferentiated pluripotent cells in the course of development.
      Therefore, TIF1alpha could belong to a novel class of chromatin-associated TIFs
      that facilitate the access of transregulators (e.g. liganded nuclear receptors)
      to their cognate sites in target genes, thereby participitating in the epigenetic
      control of transcription during embryonic development and cell differentiation.
FAU - Remboutsika, E
AU  - Remboutsika E
AD  - Institut de Genetique et de Biologie Moleculaire et Cellulaire (IGBMC),
      CNRS/INSERM/ULP/, College de France, BP 163, France.
FAU - Lutz, Y
AU  - Lutz Y
FAU - Gansmuller, A
AU  - Gansmuller A
FAU - Vonesch, J L
AU  - Vonesch JL
FAU - Losson, R
AU  - Losson R
FAU - Chambon, P
AU  - Chambon P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Chromatin)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (Euchromatin)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Transcription Factors)
RN  - 0 (transcriptional intermediary factor 1)
RN  - EC 3.1.31.1 (Micrococcal Nuclease)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/metabolism
MH  - Chromatin/*metabolism
MH  - Chromosomal Proteins, Non-Histone/*metabolism
MH  - Drosophila
MH  - Euchromatin
MH  - Interphase
MH  - Metaphase
MH  - Mice
MH  - Micrococcal Nuclease/metabolism
MH  - Nuclear Proteins/*metabolism
MH  - Receptors, Cytoplasmic and Nuclear/*metabolism
MH  - Transcription Factors/*metabolism
MH  - Xenopus
EDAT- 1999/05/13 00:00
MHDA- 1999/05/13 00:01
CRDT- 1999/05/13 00:00
PHST- 1999/05/13 00:00 [pubmed]
PHST- 1999/05/13 00:01 [medline]
PHST- 1999/05/13 00:00 [entrez]
PST - ppublish
SO  - J Cell Sci. 1999 Jun;112 ( Pt 11):1671-83.