PMID- 10235641
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20190905
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 407
IP  - 4
DP  - 1999 May 17
TI  - CART peptide-immunoreactive neurones in the nucleus accumbens in monkeys:
      ultrastructural analysis, colocalization studies, and synaptic interactions with 
      dopaminergic afferents.
PG  - 491-511
AB  - Cocaine- and amphetamine-regulated transcript (CART) is a novel mRNA whose level 
      of expression was found to be increased in the striatum after acute
      administration of psychomotor stimulants in rats. To define better the potential 
      role of CART peptides in behavioural and physiologic changes induced by
      psychomotor stimulants, we analyzed the distribution, ultrastructural features,
      synaptic connectivity, and transmitter content of CART peptide-immunoreactive
      neurones in the nucleus accumbens in monkeys. Medium-sized CART
      peptide-immunoreactive neurones within a rich plexus of labelled varicosities
      were found mostly in the medial division of the shell of the nucleus accumbens in
      monkeys. At the electron microscope level, CART peptide immunoreactivity was
      exclusively associated with neuronal structures that included perikarya,
      dendrites, spines as well as nerve terminals packed with electron-lucent and
      dense-core vesicles. Most CART peptide-containing somata displayed the
      ultrastructural features of striatal output neurones. The majority of labelled
      terminals formed symmetric axodendritic synapses and displayed gamma-aminobutyric
      acid (GABA) immunoreactivity. CART peptide-immunoreactive somata were not
      immunoreactive for parvalbumin and somatostatin, two markers of striatal
      interneurones, nor for calbindin D-28k, a marker of a subpopulation of projection
      neurones. In double-immunostained sections, CART peptide-immunoreactive dendrites
      were found to be contacted by tyrosine hydroxylase-positive terminals which
      displayed the ultrastructural features of dopamine-containing boutons. These
      findings strongly suggest that CART peptides may be a cotransmitter with GABA in 
      a subpopulation of projection neurones in the monkey accumbens. Furthermore, the 
      fact that CART peptide-immunoreactive neurones receive direct synaptic inputs
      from dopaminergic afferents and are particularly abundant in the caudomedial
      division of the shell of the nucleus accumbens suggest that CART peptides might
      be involved in neuronal and behavioural changes that underlie addiction to
      psychomotor stimulants and feeding in primates.
FAU - Smith, Y
AU  - Smith Y
AD  - Yerkes Regional Primate Research Center, Department of Neurology, Emory
      University, Atlanta, Georgia 30322, USA. yoland@rmy.emory.edu
FAU - Kieval, J
AU  - Kieval J
FAU - Couceyro, P R
AU  - Couceyro PR
FAU - Kuhar, M J
AU  - Kuhar MJ
LA  - eng
GR  - DA 10732/DA/NIDA NIH HHS/United States
GR  - RR 00165/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Calbindins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Parvalbumins)
RN  - 0 (S100 Calcium Binding Protein G)
RN  - 0 (cocaine- and amphetamine-regulated transcript protein)
RN  - 51110-01-1 (Somatostatin)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Calbindins
MH  - Dopamine/metabolism
MH  - Female
MH  - Immunohistochemistry
MH  - Macaca mulatta
MH  - Male
MH  - Microscopy, Electron
MH  - Nerve Tissue Proteins/*metabolism
MH  - Neurons/*metabolism/ultrastructure
MH  - Neurons, Afferent/physiology
MH  - Nucleus Accumbens/cytology/*metabolism/ultrastructure
MH  - Parvalbumins/metabolism
MH  - Primates/*metabolism
MH  - S100 Calcium Binding Protein G/metabolism
MH  - Saimiri
MH  - Somatostatin/metabolism
MH  - Synapses/physiology
MH  - Tissue Distribution/physiology
MH  - Tyrosine 3-Monooxygenase/metabolism
MH  - gamma-Aminobutyric Acid/metabolism
EDAT- 1999/05/11 02:03
MHDA- 2000/06/20 09:00
CRDT- 1999/05/11 02:03
PHST- 1999/05/11 02:03 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/05/11 02:03 [entrez]
AID - 10.1002/(SICI)1096-9861(19990517)407:4<491::AID-CNE3>3.0.CO;2-0 [pii]
AID - 10.1002/(sici)1096-9861(19990517)407:4<491::aid-cne3>3.0.co;2-0 [doi]
PST - ppublish
SO  - J Comp Neurol. 1999 May 17;407(4):491-511. doi:
      10.1002/(sici)1096-9861(19990517)407:4<491::aid-cne3>3.0.co;2-0.