PMID- 10235541
OWN - NLM
STAT- MEDLINE
DCOM- 19990524
LR  - 20090629
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 40
IP  - 6
DP  - 1999 May
TI  - Pancreatic lipase-related protein 1 mRNA in female mouse lacrimal gland.
PG  - 1081-90
AB  - PURPOSE: Differential display analysis was used to look for gender differences in
      lacrimal gland gene expression. The expression of a female-specific mouse
      lacrimal gland mRNA that encoded pancreatic lipase-related protein 1 (PLRP1) was 
      identified and characterized. METHODS: Differential display analysis of the
      exorbital lacrimal glands of male and female Swiss Webster mice detected a
      potential female-specific cDNA, designated Y2. Using the technique of rapid
      amplification of cDNA ends, a full-length cDNA of Y2 was obtained and the
      nucleotide sequence determined. To assess tissue-specific expression, a labeled
      Y2 cDNA probe was hybridized to RNA blots of male and female mouse lacrimal,
      harderian, parotid, mandibular, sublingual, and pancreas glands and liver. Y2
      cDNA was also hybridized to RNA blots of male and female rat lacrimal gland and
      male rat pancreas. To determine subcellular localization, Y2 sense and antisense 
      RNA probes were hybridized to female mouse lacrimal gland frozen sections.
      RESULTS: GenBank database sequence comparisons indicated that Y2 encoded mouse
      PLRP1. RNA blots documented that PLRP1 was expressed in female, but not in male, 
      mouse lacrimal gland. PLRP1 mRNA was also expressed in male and female mouse
      sublingual gland and pancreas. Expression of PLRP1 was not detected in male or
      female rat lacrimal gland. In situ hybridization showed that PLRP1 was expressed 
      in the acinar cells of the female mouse lacrimal gland. CONCLUSIONS: Lacrimal
      gland expression of PLRP1 mRNA was gender and species specific. Female, but not
      male, mouse lacrimal gland expressed PLRPI mRNA. Neither female nor male rat
      lacrimal gland expressed PLRP1 mRNA. PLRP1 protein may be secreted in mouse
      tears, where it may function as a lipolytic enzyme, modifying tear film lipids.
FAU - Remington, S G
AU  - Remington SG
AD  - HealthPartners Research Foundation, St. Paul, Minnesota, USA.
FAU - Lima, P H
AU  - Lima PH
FAU - Nelson, J D
AU  - Nelson JD
LA  - eng
SI  - GENBANK/AF061274
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - EC 3.1.1.3 (Lipase)
RN  - EC 3.1.1.3 (pancreatic lipase-related protein 1)
SB  - IM
MH  - Amino Acid Sequence/genetics
MH  - Animals
MH  - Base Sequence/genetics
MH  - DNA, Complementary/genetics
MH  - Female
MH  - Lacrimal Apparatus/cytology/*metabolism
MH  - Lipase/*genetics
MH  - Male
MH  - Mice/*metabolism
MH  - Molecular Sequence Data
MH  - Polymorphism, Genetic/genetics
MH  - RNA, Messenger/*metabolism
MH  - Rats/metabolism
MH  - Rats, Sprague-Dawley
MH  - *Sex Characteristics
MH  - Sublingual Gland/metabolism
EDAT- 1999/05/11 00:00
MHDA- 1999/05/11 00:01
CRDT- 1999/05/11 00:00
PHST- 1999/05/11 00:00 [pubmed]
PHST- 1999/05/11 00:01 [medline]
PHST- 1999/05/11 00:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 1999 May;40(6):1081-90.