PMID- 10235267
OWN - NLM
STAT- MEDLINE
DCOM- 19990614
LR  - 20091119
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 398
IP  - 6730
DP  - 1999 Apr 29
TI  - Composite co-activator ARC mediates chromatin-directed transcriptional
      activation.
PG  - 828-32
AB  - Gene activation in eukaryotes is regulated by complex mechanisms in which the
      recruitment and assembly of the transcriptional machinery is directed by gene-
      and cell-type-specific DNA-binding proteins. When DNA is packaged into chromatin,
      the regulation of gene activation requires new classes of chromatin-targeting
      activity. In humans, a multisubunit cofactor functions in a chromatin-selective
      manner to potentiate synergistic gene activation by the transcriptional
      activators SREBP-1a and Sp1. Here we show that this activator-recruited cofactor 
      (ARC) interacts directly with several different activators, including SREBP-1a,
      VP16 and the p65 subunit of NF-kappaB, and strongly enhances transcription
      directed by these activators in vitro with chromatin-assembled DNA templates. The
      ARC complex consists of 16 or more subunits; some of these are novel gene
      products, whereas others are present in other multisubunit cofactors, such as
      CRSP, NAT and mammalian Mediator. Detailed analysis indicates that the ARC
      complex is probably identical to the nuclear hormone-receptor cofactor DRIP.
      Thus, ARC/DRIP is a large composite co-activator that belongs to a family of
      related cofactors and is targeted by different classes of activator to mediate
      transcriptional stimulation.
FAU - Naar, A M
AU  - Naar AM
AD  - Howard Hughes Medical Institute, Department of Molecular and Cell Biology,
      University of California, Berkeley 94720, USA.
FAU - Beaurang, P A
AU  - Beaurang PA
FAU - Zhou, S
AU  - Zhou S
FAU - Abraham, S
AU  - Abraham S
FAU - Solomon, W
AU  - Solomon W
FAU - Tjian, R
AU  - Tjian R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (CRSP protein, human)
RN  - 0 (Chromatin)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Herpes Simplex Virus Protein Vmw65)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Med17 protein, human)
RN  - 0 (Mediator Complex)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, LDL)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (SREBF1 protein, human)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (Sterol Regulatory Element Binding Protein 1)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *CCAAT-Enhancer-Binding Proteins
MH  - Chromatin/*physiology
MH  - DNA-Binding Proteins/metabolism
MH  - Escherichia coli
MH  - HeLa Cells
MH  - Herpes Simplex Virus Protein Vmw65/metabolism
MH  - Humans
MH  - Macromolecular Substances
MH  - Mediator Complex
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/metabolism
MH  - Receptors, LDL/genetics
MH  - Recombinant Fusion Proteins
MH  - Sp1 Transcription Factor/metabolism
MH  - Sterol Regulatory Element Binding Protein 1
MH  - Trans-Activators/chemistry
MH  - Transcription Factors/chemistry/metabolism
MH  - *Transcriptional Activation
EDAT- 1999/05/11 02:03
MHDA- 2001/03/23 10:01
CRDT- 1999/05/11 02:03
PHST- 1999/05/11 02:03 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/05/11 02:03 [entrez]
AID - 10.1038/19789 [doi]
PST - ppublish
SO  - Nature. 1999 Apr 29;398(6730):828-32. doi: 10.1038/19789.