PMID- 10235110
OWN - NLM
STAT- MEDLINE
DCOM- 19990528
LR  - 20061019
IS  - 1044-5498 (Print)
IS  - 1044-5498 (Linking)
VI  - 18
IP  - 4
DP  - 1999 Apr
TI  - Genomic organization of the genes for human and mouse CC chemokine LEC.
PG  - 275-83
AB  - Liver-expressed chemokine (LEC) is a CC chemokine that is selectively expressed
      in the liver. We report here the structures of the human and mouse genes for LEC.
      The human LEC gene (SCYA16) was isolated from a bacterial artificial chromosome
      (BAC) clone that also contained CC chemokine genes for MPIF-1/Ckbeta8,
      HCC-2/Lkn-1/MIP-5/MIP-1delta, and HCC-1. The LEC gene is approximately 5.0 kb in 
      length and has a three-exon and two-intron structure common to most CC chemokine 
      genes. However, the promoter region is devoid of a typical TATA box, and
      transcription initiates at multiple sites. The gene for CC chemokine HCC-1, which
      is most similar to LEC, is located approximately 2.2 kb upstream from the 5' end 
      of the LEC gene in a head-to-tail fashion. The mouse DNA fragment that hybridized
      with the human LEC cDNA was isolated from a BAC clone that also contained the CC 
      chemokine genes for C10, MRP-2/CCF18/MIP-1gamma, and RANTES. Sequence analysis
      revealed that the isolated gene does not encode a functional chemokine because of
      deletions, insertions, and base changes. Southern blot analysis revealed that the
      sequence isolated from the BAC clone was the only one hybridizing with human LEC 
      cDNA in the mouse genome. Therefore, mice may have only an LEC pseudogene.
FAU - Fukuda, S
AU  - Fukuda S
AD  - Department of Biochemistry, Kumamoto University Medical School, Honjo, Japan.
FAU - Hanano, Y
AU  - Hanano Y
FAU - Iio, M
AU  - Iio M
FAU - Miura, R
AU  - Miura R
FAU - Yoshie, O
AU  - Yoshie O
FAU - Nomiyama, H
AU  - Nomiyama H
LA  - eng
SI  - GENBANK/AB018249
SI  - GENBANK/AB018250
PT  - Journal Article
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (CCL16 protein, human)
RN  - 0 (Chemokines, CC)
RN  - 0 (DNA, Complementary)
SB  - IM
MH  - Adolescent
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chemokines, CC/*genetics
MH  - DNA, Complementary
MH  - Guinea Pigs
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Tumor Cells, Cultured
EDAT- 1999/05/11 00:00
MHDA- 1999/05/11 00:01
CRDT- 1999/05/11 00:00
PHST- 1999/05/11 00:00 [pubmed]
PHST- 1999/05/11 00:01 [medline]
PHST- 1999/05/11 00:00 [entrez]
AID - 10.1089/104454999315330 [doi]
PST - ppublish
SO  - DNA Cell Biol. 1999 Apr;18(4):275-83. doi: 10.1089/104454999315330.