PMID- 10234569
OWN - NLM
STAT- MEDLINE
DCOM- 19990706
LR  - 20091119
IS  - 0884-0431 (Print)
IS  - 0884-0431 (Linking)
VI  - 14
IP  - 4
DP  - 1999 Apr
TI  - Characterization of mouse cathepsin K gene, the gene promoter, and the gene
      expression.
PG  - 487-99
AB  - Cathepsin K, a lysosomal cysteine protease, is abundantly and selectively
      expressed in osteoclasts and has a specialized role in osteoclast-mediated bone
      resorption. In contrast to function studies, transcription regulation of
      cathepsin K remains largely unknown. In this study, the gene encoding mouse
      cathepsin K and the promoter have been isolated and completely sequenced. In
      addition, the temporal and spatial expressions of cathepsin K have been
      characterized. Intrachromosomal mapping studies revealed that the gene contains
      eight exons and seven introns spanning approximately 10.6 kb of genomic DNA, a
      genomic organization that was highly conserved with respect to its human
      homology. Analysis of the 9 kb 5' flanking region indicates that this gene lacks 
      canonical TATA and CAAT boxes and contains multiple putative transcription
      regulatory elements which are also present in the comparable position of 5'
      flanking region of human cathepsin K gene. Mouse cathepsin K was found to be a
      single-copy gene. Northern blot analysis of RNAs from a number of mouse tissues
      revealed that cathepsin K mRNA is selectively expressed in osteoclast. The
      selective expression of cathepsin K was confirmed by anticathepsin K
      immunohistochemical staining. The sequence of cathepsin K expression was linked
      to osteoclast differentiation in vivo and in vitro by a tartrate-resistant acid
      phosphatase-anticathepsin K dual immunostaining technique. Cathepsin K is
      initially expressed at the preosteoclast stage and throughout the mature
      osteoclast stage. The primer extension assay indicated a major transcription
      start site 58 bp upstream of the initiator Met codon. The characterization of the
      cathepsin K gene, its promoter, and the temporal and spatial expression may
      provide valuable insights into its osteoclast-specific expression and the
      molecular mechanisms responsible for osteoclast activation.
FAU - Li, Y P
AU  - Li YP
AD  - Department of Cytokine Biology, Forsyth Dental Center, Boston, Massachusetts,
      USA.
FAU - Chen, W
AU  - Chen W
LA  - eng
GR  - DE-10887/DE/NIDCR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Bone Miner Res
JT  - Journal of bone and mineral research : the official journal of the American
      Society for Bone and Mineral Research
JID - 8610640
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Messenger)
RN  - 9007-49-2 (DNA)
RN  - EC 3.4.- (Cathepsins)
RN  - EC 3.4.22.38 (CTSK protein, human)
RN  - EC 3.4.22.38 (Cathepsin K)
RN  - EC 3.4.22.38 (Ctsk protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cathepsin K
MH  - Cathepsins/*genetics
MH  - Cell Differentiation
MH  - Cloning, Molecular
MH  - DNA/genetics
MH  - DNA Primers/genetics
MH  - Exons
MH  - Gene Expression
MH  - Humans
MH  - Introns
MH  - Mice
MH  - Molecular Sequence Data
MH  - Osteoclasts/cytology/enzymology
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics/metabolism
MH  - Restriction Mapping
MH  - Sequence Homology, Nucleic Acid
MH  - Species Specificity
EDAT- 1999/05/11 00:00
MHDA- 1999/05/11 00:01
CRDT- 1999/05/11 00:00
PHST- 1999/05/11 00:00 [pubmed]
PHST- 1999/05/11 00:01 [medline]
PHST- 1999/05/11 00:00 [entrez]
AID - 10.1359/jbmr.1999.14.4.487 [doi]
PST - ppublish
SO  - J Bone Miner Res. 1999 Apr;14(4):487-99. doi: 10.1359/jbmr.1999.14.4.487.