PMID- 10234050
OWN - NLM
STAT- MEDLINE
DCOM- 20000522
LR  - 20191023
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 19
IP  - 10
DP  - 1999 May 15
TI  - Simultaneous binding of two protein kinases to a calcium-dependent potassium
      channel.
PG  - RC4
AB  - Large-conductance calcium-dependent potassium channels are subject to modulation 
      by protein kinases, phosphatases, and other signaling proteins, and it has been
      inferred from electrophysiological experiments that signaling proteins sometimes 
      can be intimately associated with these channels in a regulatory complex. We show
      here that endogenous protein kinase activity coimmunoprecipitates with both
      native and recombinant Drosophila Slowpoke (dSlo) calcium-dependent potassium
      channels. Coimmunoprecipitation experiments using antibodies against several
      protein kinases demonstrate that dSlo can bind simultaneously to the Src tyrosine
      kinase and to the catalytic subunit of the cAMP-dependent protein kinase (PKAc). 
      Both kinases can phosphorylate the channel in Drosophila heads and in
      heterologous host cells. The PKAc binds directly to a 172-amino acid region in
      the C-terminal domain of dSlo, without the intervention of regulatory subunits or
      anchoring proteins, and channel phosphorylation by PKAc is not required for this 
      binding interaction. In contrast, several phosphorylatable tyrosine residues in
      dSlo are important for Src binding. The results are consistent with the idea that
      an ion channel can act as a scaffold for its own specific set of modulatory
      enzymes.
FAU - Wang, J
AU  - Wang J
AD  - Department of Biochemistry and Volen Center for Complex Systems, Brandeis
      University, Waltham, Massachusetts 02454, USA.
FAU - Zhou, Y
AU  - Zhou Y
FAU - Wen, H
AU  - Wen H
FAU - Levitan, I B
AU  - Levitan IB
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for
      Neuroscience
JID - 8102140
RN  - 0 (Drosophila Proteins)
RN  - 0 (Large-Conductance Calcium-Activated Potassium Channels)
RN  - 0 (Potassium Channels)
RN  - 0 (Potassium Channels, Calcium-Activated)
RN  - 0 (Recombinant Proteins)
RN  - 0 (slo protein, Drosophila)
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Calcium/*metabolism
MH  - Cell Line
MH  - Cyclic AMP-Dependent Protein Kinases/*metabolism
MH  - Drosophila
MH  - Drosophila Proteins
MH  - Large-Conductance Calcium-Activated Potassium Channels
MH  - Phosphorylation
MH  - Potassium Channels/*metabolism
MH  - *Potassium Channels, Calcium-Activated
MH  - Precipitin Tests
MH  - Recombinant Proteins/metabolism
MH  - Transfection
MH  - src-Family Kinases/*metabolism
PMC - PMC6782707
EDAT- 1999/05/11 00:00
MHDA- 2000/06/08 00:00
CRDT- 1999/05/11 00:00
PHST- 1999/05/11 00:00 [pubmed]
PHST- 2000/06/08 00:00 [medline]
PHST- 1999/05/11 00:00 [entrez]
PST - ppublish
SO  - J Neurosci. 1999 May 15;19(10):RC4.