PMID- 10234023
OWN - NLM
STAT- MEDLINE
DCOM- 19990601
LR  - 20191023
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 19
IP  - 10
DP  - 1999 May 15
TI  - Postsynaptic density-93 interacts with the delta2 glutamate receptor subunit at
      parallel fiber synapses.
PG  - 3926-34
AB  - The glutamate receptor subunit delta2 has a unique distribution at the parallel
      fiber-Purkinje cell synapse of the cerebellum, which is developmentally regulated
      such that delta2 occurs at both parallel fiber synapses and climbing fiber
      synapses early in development but is restricted to parallel fiber synapses in
      adult animals. To identify proteins that might be involved in the trafficking or 
      docking of delta2 receptors, we screened a yeast two-hybrid library with the
      cytosolic C terminus of delta2 and isolated a member of the postsynaptic density 
      (PSD)-95 family of proteins, which are known to interact with the extreme C
      termini of NMDA receptors. We find that delta2 binds specifically to PSD-93,
      which is enriched in Purkinje cells. In addition, PSD-93 clusters delta2 when
      they are coexpressed in heterologous cells, and clustering is disrupted by point 
      mutations of delta2 that disrupt the delta2-PSD-93 interaction. Ultrastructural
      localization of PSD-93 and delta2 shows they are colocalized at parallel fiber
      synapses; however, PSD-93 also is present at climbing fiber synapses of the adult
      rat, where delta2 is not found, indicating that the presence of PSD-93 alone is
      not sufficient for determining the synaptic expression of delta2.
FAU - Roche, K W
AU  - Roche KW
AD  - Laboratory of Neurochemistry, National Institute on Deafness and Other
      Communication Disorders, National Institutes of Health, Bethesda, Maryland 20892,
      USA. rochek@nidcd.nih.gov
FAU - Ly, C D
AU  - Ly CD
FAU - Petralia, R S
AU  - Petralia RS
FAU - Wang, Y X
AU  - Wang YX
FAU - McGee, A W
AU  - McGee AW
FAU - Bredt, D S
AU  - Bredt DS
FAU - Wenthold, R J
AU  - Wenthold RJ
LA  - eng
GR  - GM36017/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for
      Neuroscience
JID - 8102140
RN  - 0 (Disks Large Homolog 4 Protein)
RN  - 0 (Dlg4 protein, rat)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Mpp2 protein, rat)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, Glutamate)
RN  - 0 (glutamate receptor delta 2)
RN  - 0 (postsynaptic density proteins)
SB  - IM
MH  - Animals
MH  - Brain/metabolism/ultrastructure
MH  - Cell Line
MH  - Disks Large Homolog 4 Protein
MH  - Gene Library
MH  - HeLa Cells
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Membrane Proteins
MH  - Nerve Fibers/*metabolism
MH  - Nerve Tissue Proteins/*metabolism
MH  - Rats
MH  - Receptors, Glutamate/*metabolism
MH  - Synapses/*metabolism
PMC - PMC6782719
EDAT- 1999/05/11 00:00
MHDA- 1999/05/11 00:01
CRDT- 1999/05/11 00:00
PHST- 1999/05/11 00:00 [pubmed]
PHST- 1999/05/11 00:01 [medline]
PHST- 1999/05/11 00:00 [entrez]
PST - ppublish
SO  - J Neurosci. 1999 May 15;19(10):3926-34.