PMID- 10233994
OWN - NLM
STAT- MEDLINE
DCOM- 19990607
LR  - 20181113
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 73
IP  - 6
DP  - 1999 Jun
TI  - An orphan G protein-coupled receptor, GPR1, acts as a coreceptor to allow
      replication of human immunodeficiency virus types 1 and 2 in brain-derived cells.
PG  - 5231-9
AB  - Twelve G protein-coupled receptors, including chemokine receptors, act as
      coreceptors and determinants for the cell tropisms of human immunodeficiency
      virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus (SIV). We isolated
      HIV-1 variants from T-cell-line (T)- and macrophage (M)-tropic (i.e., dualtropic)
      (R5-R3-X4) HIV-1 strains and also produced six HIV-1 mutants carrying
      single-point amino acid substitutions at the tip of the V3 region of the Env
      protein of HIV-1. These variants and three mutants infected brain-derived
      CD4-positive cells that are resistant to M-, T-, or dualtropic (R5, X4, or R5-X4)
      HIV-1 strains. However, a factor that determines this cell tropism has not been
      identified. This study shows that primary brain-derived fibroblast-like cell
      strains, BT-3 and BT-20/N, as well as a CD4-transduced glioma cell line, U87/CD4,
      which were susceptible to these HIV-1 variants and mutants and the HIV-2ROD
      strain, expressed mRNA of an orphan G protein-coupled receptor (GPCR), GPR1. When
      a CD4-positive cell line which was strictly resistant to infection with diverse
      HIV-1 and HIV-2 strains was transduced with GPR1, the cell line became
      susceptible to these HIV-1 variants and mutants and to an HIV-2 strain but not to
      T- or dualtropic HIV-1 strains, and numerous syncytia formed after infection.
      These results indicate that GPR1 functions as a coreceptor for the HIV-1 variants
      and mutants and for the HIV-2ROD strain in vitro.
FAU - Shimizu, N
AU  - Shimizu N
AD  - Department of Hygiene and Virology, Gunma University School of Medicine, 3-39-22 
      Showa-machi, Maebashi, Gunma 371-8511, Minato-ku, Tokyo 105-0071, Japan.
FAU - Soda, Y
AU  - Soda Y
FAU - Kanbe, K
AU  - Kanbe K
FAU - Liu, H Y
AU  - Liu HY
FAU - Jinno, A
AU  - Jinno A
FAU - Kitamura, T
AU  - Kitamura T
FAU - Hoshino, H
AU  - Hoshino H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (GPR1 protein, S cerevisiae)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, HIV)
RN  - 0 (Saccharomyces cerevisiae Proteins)
SB  - IM
SB  - X
MH  - Brain/virology
MH  - Cell Line
MH  - Glioma/virology
MH  - HIV-1/*physiology
MH  - HIV-2/*physiology
MH  - Humans
MH  - Receptors, Cell Surface/*physiology
MH  - *Receptors, G-Protein-Coupled
MH  - Receptors, HIV/*physiology
MH  - *Saccharomyces cerevisiae Proteins
MH  - Structure-Activity Relationship
MH  - *Virus Replication
PMC - PMC112576
EDAT- 1999/05/11 00:00
MHDA- 1999/05/11 00:01
CRDT- 1999/05/11 00:00
PHST- 1999/05/11 00:00 [pubmed]
PHST- 1999/05/11 00:01 [medline]
PHST- 1999/05/11 00:00 [entrez]
PST - ppublish
SO  - J Virol. 1999 Jun;73(6):5231-9.