PMID- 10232922
OWN - NLM
STAT- MEDLINE
DCOM- 19990503
LR  - 20091119
IS  - 0907-4449 (Print)
IS  - 0907-4449 (Linking)
VI  - 55
IP  - Pt 1
DP  - 1999 Jan
TI  - Expression, purification and crystallization of a BH domain from the GTPase
      regulatory protein associated with focal adhesion kinase.
PG  - 356-9
AB  - Signaling by small GTPases is down-regulated by GTPase activating proteins (GAPs)
      which enhance the rate of GTP hydrolysis. The activity of GAPs specific for Rho
      GTPases resides in the BH domain, many homologues of which are found in any
      mammalian genome. One of them was identified in the GTPase regulator associated
      with focal-adhesion kinase (GRAF). It shares approximately 20% sequence identity 
      with p50RhoGAP. This GAP activates RhoA and Cdc42Hs, but not Rac. In order to
      dissect the molecular basis of this specificity, a 231-residue-long fragment
      corresponding to the BH domain of GRAF has been expressed, purified and
      crystallized. Trigonal crystals, of space group P3(1)21 or P3(2)21, with
      unit-cell dimensions a = b = 63.5, c = 90.38 A were grown from solutions of PEG
      6000. Data to 2.15 A were collected from a flash-frozen sample on an R-AXIS IV
      imaging-plate detector mounted on a rotating anode X-ray generator.
FAU - Sheffield, P J
AU  - Sheffield PJ
AD  - Department of Physiology, University of Virginia, Charlottesville 22908, USA.
FAU - Derewenda, U
AU  - Derewenda U
FAU - Taylor, J
AU  - Taylor J
FAU - Parsons, T J
AU  - Parsons TJ
FAU - Derewenda, Z S
AU  - Derewenda ZS
LA  - eng
GR  - 2 PO1 H148807 06A1/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 19990101
PL  - United States
TA  - Acta Crystallogr D Biol Crystallogr
JT  - Acta crystallographica. Section D, Biological crystallography
JID - 9305878
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (GTPase-Activating Proteins)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Focal Adhesion Protein-Tyrosine Kinases)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Adhesion Molecules/chemistry
MH  - Crystallization
MH  - Escherichia coli/genetics
MH  - Focal Adhesion Protein-Tyrosine Kinases
MH  - GTP Phosphohydrolases/*chemistry/genetics/*isolation & purification
MH  - GTPase-Activating Proteins
MH  - Gene Expression
MH  - Molecular Sequence Data
MH  - Protein-Tyrosine Kinases/chemistry
MH  - Proteins/*chemistry/genetics/*isolation & purification
MH  - Recombinant Fusion Proteins/chemistry/genetics/isolation & purification
MH  - Sequence Homology, Amino Acid
EDAT- 1999/05/08 02:12
MHDA- 2000/06/23 11:00
CRDT- 1999/05/08 02:12
PHST- 1998/04/15 00:00 [received]
PHST- 1998/07/01 00:00 [accepted]
PHST- 1999/05/08 02:12 [pubmed]
PHST- 2000/06/23 11:00 [medline]
PHST- 1999/05/08 02:12 [entrez]
AID - 10.1107/S0907444998009032 [doi]
AID - S0907444998009032 [pii]
PST - ppublish
SO  - Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):356-9. doi:
      10.1107/S0907444998009032. Epub 1999 Jan 1.