PMID- 10231361
OWN - NLM
STAT- MEDLINE
DCOM- 19990621
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 262
IP  - 1
DP  - 1999 May
TI  - Structure and splice products of the human gene encoding sds22, a putative
      mitotic regulator of protein phosphatase-1.
PG  - 36-42
AB  - sds22 is a regulatory subunit of protein phosphatase-1 that is required for the
      completion of mitosis in yeast. It consists largely of 11 tandem leucine-rich
      repeats of 22 residues that are expected to mediate interactions with other
      polypeptides, including protein phosphatase-1. In this paper, we report on the
      structure of the human gene encoding sds22, designated PPP1R7. This gene (33 kb) 
      comprises 11 exons, but these do not coincide with the sequences encoding the
      leucine-rich repeats. Up to six splice variants can be generated by exon skipping
      and alternative polyadenylation, as revealed by expressed sequence tag database
      analysis, RT-PCR and Northern blot analysis. The sds22 transcripts are expected
      to encode four different polypeptides. sds22alpha1 corresponds to the variant
      cloned previously from human brain [Renouf et al. (1995) FEBS Lett. 375, 75-78]. 
      Sds22beta1 is truncated within the ninth repeat and has a short and different
      C-terminus. Both variants also exist without the sequence corresponding to exon
      2, and these are termed sds22alpha2 and sds22beta2. The 5'-flanking region of
      PPP1R7 contains two NF-Y-binding CCAAT boxes near the transcription start site
      and potential binding sites for the transcription factors c-Myb, Ik-2 and NF-1,
      which are conserved in the mouse gene.
FAU - Ceulemans, H
AU  - Ceulemans H
AD  - Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven,
      Belgium.
FAU - Van Eynde, A
AU  - Van Eynde A
FAU - Perez-Callejon, E
AU  - Perez-Callejon E
FAU - Beullens, M
AU  - Beullens M
FAU - Stalmans, W
AU  - Stalmans W
FAU - Bollen, M
AU  - Bollen M
LA  - eng
SI  - GENBANK/AF067129
SI  - GENBANK/AF067130
SI  - GENBANK/AF067131
SI  - GENBANK/AF067132
SI  - GENBANK/AF067133
SI  - GENBANK/AF067134
SI  - GENBANK/AF067135
SI  - GENBANK/AF067136
SI  - GENBANK/AF067137
SI  - GENBANK/AH007598
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Nuclear Proteins)
RN  - 0 (PPP1R7 protein, human)
RN  - 0 (Ppp1r7 protein, mouse)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
RN  - EC 3.1.3.16 (Protein Phosphatase 1)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Cycle Proteins/*genetics
MH  - Cell Line
MH  - Chromosome Mapping
MH  - DNA Primers
MH  - DNA, Complementary
MH  - Exons
MH  - Humans
MH  - Introns
MH  - Mice
MH  - Mitosis/*genetics
MH  - Molecular Sequence Data
MH  - Nuclear Proteins
MH  - Phosphoprotein Phosphatases/*metabolism
MH  - Protein Phosphatase 1
MH  - *RNA Splicing
MH  - Sequence Homology, Nucleic Acid
EDAT- 1999/05/07 00:00
MHDA- 1999/05/07 00:01
CRDT- 1999/05/07 00:00
PHST- 1999/05/07 00:00 [pubmed]
PHST- 1999/05/07 00:01 [medline]
PHST- 1999/05/07 00:00 [entrez]
AID - 10.1046/j.1432-1327.1999.00344.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 May;262(1):36-42. doi: 10.1046/j.1432-1327.1999.00344.x.