PMID- 10231028 OWN - NLM STAT- MEDLINE DCOM- 19990720 LR - 20190513 IS - 1340-2838 (Print) IS - 1340-2838 (Linking) VI - 6 IP - 1 DP - 1999 Feb 26 TI - Characterization of a 1200-kb genomic segment of chromosome 3p22-p21.3. PG - 37-44 AB - We previously determined the nucleotide sequence and characterized the 685-kb proximal half of CEPH YAC936c1, which corresponds to a portion of human chromosome 3p21.3. In the study reported here, we characterized the remaining 515-kb of this YAC clone corresponding to the telomeric half of its human insert. The newly sequenced region contained a total of ten genes including six reported previously: phospholipase C delta 1 (PLCD1), human activin receptor type IIB (hActR-IIB), organic cation transporter-like 1 (OCTL1), organic cation transporter-like 2 (OCTL2), oxidative stress response 1 (OSR1), and human xylulokinase-like protein (XYLB). The remaining four genes present in the telomeric region included two known genes, MyD88 and ACAA, and two novel genes. One (designated ENGL) of the novel sequences was found to encode an amino-acid sequence homologous to the family of DNA/RNA endonucleases, especially endonuclease G. The other gene F56 revealed no significant homology to any known genes. These results disclosed complete physical and transcriptional maps of the 1200-kb region of 3p present in YAC 936c1. FAU - Daigo, Y AU - Daigo Y AD - Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Japan. FAU - Isomura, M AU - Isomura M FAU - Nishiwaki, T AU - Nishiwaki T FAU - Tamari, M AU - Tamari M FAU - Ishikawa, S AU - Ishikawa S FAU - Kai, M AU - Kai M FAU - Murata, Y AU - Murata Y FAU - Takeuchi, K AU - Takeuchi K FAU - Yamane, Y AU - Yamane Y FAU - Hayashi, R AU - Hayashi R FAU - Minami, M AU - Minami M FAU - Fujino, M A AU - Fujino MA FAU - Hojo, Y AU - Hojo Y FAU - Uchiyama, I AU - Uchiyama I FAU - Takagi, T AU - Takagi T FAU - Nakamura, Y AU - Nakamura Y LA - eng SI - GENBANK/AB020523 SI - GENBANK/AB020735 PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - DNA Res JT - DNA research : an international journal for rapid publication of reports on genes and genomes JID - 9423827 SB - IM MH - Amino Acid Sequence MH - Blotting, Northern MH - Chromosomes, Artificial, Yeast MH - *Chromosomes, Human, Pair 3 MH - Gene Library MH - Humans MH - In Situ Hybridization, Fluorescence MH - Models, Genetic MH - Molecular Sequence Data MH - Physical Chromosome Mapping MH - Sequence Analysis, DNA MH - Sequence Homology, Amino Acid MH - Software EDAT- 1999/05/07 00:00 MHDA- 1999/05/07 00:01 CRDT- 1999/05/07 00:00 PHST- 1999/05/07 00:00 [pubmed] PHST- 1999/05/07 00:01 [medline] PHST- 1999/05/07 00:00 [entrez] AID - 10.1093/dnares/6.1.37 [doi] PST - ppublish SO - DNA Res. 1999 Feb 26;6(1):37-44. doi: 10.1093/dnares/6.1.37.