PMID- 10230800
OWN - NLM
STAT- MEDLINE
DCOM- 19990519
LR  - 20190822
IS  - 0896-6273 (Print)
IS  - 0896-6273 (Linking)
VI  - 22
IP  - 4
DP  - 1999 Apr
TI  - A dynamically regulated 14-3-3, Slob, and Slowpoke potassium channel complex in
      Drosophila presynaptic nerve terminals.
PG  - 809-18
AB  - Slob is a novel protein that binds to the carboxy-terminal domain of the
      Drosophila Slowpoke (dSlo) calcium-dependent potassium (K(Ca)) channel. A yeast
      two-hybrid screen with Slob as bait identifies the zeta isoform of 14-3-3 as a
      Slob-binding protein. Coimmunoprecipitation experiments from Drosophila heads and
      transfected cells confirm that 14-3-3 interacts with dSlo via Slob. All three
      proteins are colocalized presynaptically at Drosophila neuromuscular junctions.
      Two serine residues in Slob are required for 14-3-3 binding, and the binding is
      dynamically regulated in Drosophila by calcium/calmodulin-dependent kinase II
      (CaMKII) phosphorylation. 14-3-3 coexpression dramatically alters dSlo channel
      properties when wild-type Slob is present but not when a double serine mutant
      Slob that is incapable of binding 14-3-3 is present. The results provide evidence
      for a dSlo/Slob/14-3-3 regulatory protein complex.
FAU - Zhou, Y
AU  - Zhou Y
AD  - Department of Biochemistry, Volen Center for Complex Systems, Brandeis
      University, Waltham, Massachusetts 02454, USA.
FAU - Schopperle, W M
AU  - Schopperle WM
FAU - Murrey, H
AU  - Murrey H
FAU - Jaramillo, A
AU  - Jaramillo A
FAU - Dagan, D
AU  - Dagan D
FAU - Griffith, L C
AU  - Griffith LC
FAU - Levitan, I B
AU  - Levitan IB
LA  - eng
GR  - R01 GM054408/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neuron
JT  - Neuron
JID - 8809320
RN  - 0 (14-3-3 Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Potassium Channels)
RN  - 0 (Proteins)
RN  - 0 (Slob protein, Drosophila)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - 14-3-3 Proteins
MH  - Animals
MH  - Calcium/physiology
MH  - Drosophila/*physiology
MH  - *Drosophila Proteins
MH  - Hybridization, Genetic
MH  - Insect Proteins/physiology
MH  - Nerve Tissue Proteins/physiology
MH  - Neuromuscular Junction/physiology
MH  - Phosphorylation
MH  - Potassium Channels/physiology
MH  - Presynaptic Terminals/physiology
MH  - Proteins/physiology
MH  - *Tyrosine 3-Monooxygenase
EDAT- 1999/05/07 00:00
MHDA- 1999/05/07 00:01
CRDT- 1999/05/07 00:00
PHST- 1999/05/07 00:00 [pubmed]
PHST- 1999/05/07 00:01 [medline]
PHST- 1999/05/07 00:00 [entrez]
AID - S0896-6273(00)80739-4 [pii]
AID - 10.1016/s0896-6273(00)80739-4 [doi]
PST - ppublish
SO  - Neuron. 1999 Apr;22(4):809-18. doi: 10.1016/s0896-6273(00)80739-4.