PMID- 10230407
OWN - NLM
STAT- MEDLINE
DCOM- 19990527
LR  - 20190915
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 3
IP  - 4
DP  - 1999 Apr
TI  - ROC1, a homolog of APC11, represents a family of cullin partners with an
      associated ubiquitin ligase activity.
PG  - 535-41
AB  - We have identified two highly conserved RING finger proteins, ROC1 and ROC2, that
      are homologous to APC11, a subunit of the anaphase-promoting complex. ROC1 and
      ROC2 commonly interact with all cullins while APC11 specifically interacts with
      APC2, a cullin-related APC subunit. YeastROC1 encodes an essential gene whose
      reduced expression resulted in multiple, elongated buds and accumulation of Sic1p
      and Cln2p. ROC1 and APC11 immunocomplexes can catalyze isopeptide ligations to
      form polyubiquitin chains in an E1- and E2-dependent manner. ROC1 mutations
      completely abolished their ligase activity without noticeable changes in
      associated proteins. Ubiquitination of phosphorylated I kappa B alpha can be
      catalyzed by the ROC1 immunocomplex in vitro. Hence, combinations of ROC/APC11
      and cullin proteins proteins potentially constitute a wide variety of ubiquitin
      ligases.
FAU - Ohta, T
AU  - Ohta T
AD  - Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel
      Hill 27599, USA.
FAU - Michel, J J
AU  - Michel JJ
FAU - Schottelius, A J
AU  - Schottelius AJ
FAU - Xiong, Y
AU  - Xiong Y
LA  - eng
SI  - GENBANK/AF142059
SI  - GENBANK/AF142060
GR  - CA65572/CA/NCI NIH HHS/United States
GR  - CA68377/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (CDC4 protein, S cerevisiae)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cullin 1)
RN  - 0 (Cullin Proteins)
RN  - 0 (F-Box Proteins)
RN  - 0 (F-Box-WD Repeat-Containing Protein 7)
RN  - 0 (FBXW7 protein, human)
RN  - 0 (Fungal Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Ubiquitins)
RN  - EC 2.3.2.23 (Ubiquitin-Protein Ligase Complexes)
RN  - EC 2.3.2.27 (ANAPC11 protein, human)
RN  - EC 2.3.2.27 (APC2 protein, S cerevisiae)
RN  - EC 2.3.2.27 (Anaphase-Promoting Complex-Cyclosome)
RN  - EC 2.3.2.27 (Apc11 Subunit, Anaphase-Promoting Complex-Cyclosome)
RN  - EC 2.3.2.27 (Apc2 Subunit, Anaphase-Promoting Complex-Cyclosome)
RN  - EC 2.3.2.27 (SKP Cullin F-Box Protein Ligases)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 6.- (Ligases)
RN  - EC 6.3.2.- (Peptide Synthases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Anaphase-Promoting Complex-Cyclosome
MH  - Apc11 Subunit, Anaphase-Promoting Complex-Cyclosome
MH  - Apc2 Subunit, Anaphase-Promoting Complex-Cyclosome
MH  - Base Sequence
MH  - Cell Cycle Proteins/*genetics/metabolism
MH  - Cell Line
MH  - *Cullin Proteins
MH  - *F-Box Proteins
MH  - F-Box-WD Repeat-Containing Protein 7
MH  - Fungal Proteins/chemistry/*genetics
MH  - Humans
MH  - Ligases/*genetics/metabolism
MH  - Molecular Sequence Data
MH  - Peptide Synthases/*genetics/metabolism
MH  - Phenotype
MH  - SKP Cullin F-Box Protein Ligases
MH  - Saccharomyces cerevisiae/genetics
MH  - Saccharomyces cerevisiae Proteins
MH  - Sequence Homology, Amino Acid
MH  - *Ubiquitin-Protein Ligase Complexes
MH  - *Ubiquitin-Protein Ligases
MH  - Ubiquitins/metabolism
EDAT- 1999/05/07 00:00
MHDA- 1999/05/07 00:01
CRDT- 1999/05/07 00:00
PHST- 1999/05/07 00:00 [pubmed]
PHST- 1999/05/07 00:01 [medline]
PHST- 1999/05/07 00:00 [entrez]
AID - S1097-2765(00)80482-7 [pii]
AID - 10.1016/s1097-2765(00)80482-7 [doi]
PST - ppublish
SO  - Mol Cell. 1999 Apr;3(4):535-41. doi: 10.1016/s1097-2765(00)80482-7.