PMID- 10230406
OWN - NLM
STAT- MEDLINE
DCOM- 19990527
LR  - 20161124
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 3
IP  - 4
DP  - 1999 Apr
TI  - Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the
      ubiquitination of I kappa B alpha.
PG  - 527-33
AB  - Activation of the transcription factor NF-kappa B in response to proinflammatory 
      stimuli requires the phosphorylation-triggered and ubiquitin-dependent
      degradation of the NF-kappa B inhibitor, I kappa B alpha. Here, we show the in
      vitro reconstitution of the phosphorylation-dependent ubiquitination of I kappa B
      alpha with purified components. ROC1, a novel SCF-associated protein, is
      recruited by cullin 1 to form a quatemary SCFHOS-ROC1 holenzyme (with Skp1 and
      the beta-TRCP homolog HOS). SCFHOS-ROC1 binds IKK beta-phosphorylated I kappa B
      alpha and catalyzes its ubiquitination in the presence of ubiquitin, E1, and
      Cdc34. ROC1 plays a unique role in the ubiquitination reaction by
      heterodimerizing with cullin 1 to catalyze ubiquitin polymerization.
FAU - Tan, P
AU  - Tan P
AD  - Derald H. Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York, New
      York 10029, USA.
FAU - Fuchs, S Y
AU  - Fuchs SY
FAU - Chen, A
AU  - Chen A
FAU - Wu, K
AU  - Wu K
FAU - Gomez, C
AU  - Gomez C
FAU - Ronai, Z
AU  - Ronai Z
FAU - Pan, Z Q
AU  - Pan ZQ
LA  - eng
GR  - CA78419/CA/NCI NIH HHS/United States
GR  - GM55059/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cullin 1)
RN  - 0 (Cullin Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (NFKBIA protein, human)
RN  - 0 (S-Phase Kinase-Associated Proteins)
RN  - 0 (Ubiquitins)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - EC 2.3.2.27 (SKP Cullin F-Box Protein Ligases)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.10 (CHUK protein, human)
RN  - EC 2.7.11.10 (I-kappa B Kinase)
RN  - EC 2.7.11.10 (IKBKB protein, human)
RN  - EC 2.7.11.10 (IKBKE protein, human)
RN  - EC 6.3.2.- (Peptide Synthases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cell Cycle Proteins/*metabolism
MH  - *Cullin Proteins
MH  - DNA-Binding Proteins/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - I-kappa B Kinase
MH  - *I-kappa B Proteins
MH  - Molecular Sequence Data
MH  - NF-KappaB Inhibitor alpha
MH  - Peptide Synthases/*metabolism
MH  - Phosphorylation
MH  - Protein-Serine-Threonine Kinases/metabolism
MH  - S-Phase Kinase-Associated Proteins
MH  - SKP Cullin F-Box Protein Ligases
MH  - Ubiquitins/metabolism
EDAT- 1999/05/07 00:00
MHDA- 1999/05/07 00:01
CRDT- 1999/05/07 00:00
PHST- 1999/05/07 00:00 [pubmed]
PHST- 1999/05/07 00:01 [medline]
PHST- 1999/05/07 00:00 [entrez]
AID - S1097-2765(00)80481-5 [pii]
PST - ppublish
SO  - Mol Cell. 1999 Apr;3(4):527-33.