PMID- 10230404
OWN - NLM
STAT- MEDLINE
DCOM- 19990527
LR  - 20190915
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 3
IP  - 4
DP  - 1999 Apr
TI  - Ligand-independent recruitment of SRC-1 to estrogen receptor beta through
      phosphorylation of activation function AF-1.
PG  - 513-9
AB  - The estrogen receptors (ERs) alpha and beta possess a constitutive N-terminal
      activation function (AF-1) whose activity can be modulated by kinase signalling
      pathways. We demonstrate here that phosphorylation of AF-1 by MAP kinase (MAPK)
      leads to the recruitment of steroid receptor coactivator-1 (SRC-1) by ER beta in 
      vitro. Enhancement of the interaction between SRC-1 and ER beta AF-1 is also
      observed in vivo in cells either treated with EGF or expressing activated Ras.
      Two serine residues in ER beta AF-1, of which one is contained within a motif
      present in other steroid receptors, are critical for physical interaction with
      SRC-1 and transcriptional activation. Our results establish a role for nuclear
      receptor phosphorylation in the recruitment of SRC-1 and provide a molecular
      basis for ligand-independent activation by ER beta via the MAPK pathway.
FAU - Tremblay, A
AU  - Tremblay A
AD  - Molecular Oncology Group, McGill University Health Centre, Montreal, Quebec,
      Canada.
FAU - Tremblay, G B
AU  - Tremblay GB
FAU - Labrie, F
AU  - Labrie F
FAU - Giguere, V
AU  - Giguere V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Ifngr2 protein, mouse)
RN  - 0 (Ligands)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, Interferon)
RN  - 0 (Transcription Factors)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (Ncoa1 protein, mouse)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - Epidermal Growth Factor/pharmacology
MH  - Estrogen Receptor beta
MH  - Gene Expression Regulation/genetics
MH  - Genes, Reporter
MH  - Histone Acetyltransferases
MH  - Ligands
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Nuclear Receptor Coactivator 1
MH  - Phosphorylation
MH  - Receptors, Estrogen/genetics/*metabolism
MH  - Receptors, Interferon/*metabolism
MH  - Signal Transduction
MH  - Transcription Factors/*metabolism
MH  - Transfection
EDAT- 1999/05/07 00:00
MHDA- 1999/05/07 00:01
CRDT- 1999/05/07 00:00
PHST- 1999/05/07 00:00 [pubmed]
PHST- 1999/05/07 00:01 [medline]
PHST- 1999/05/07 00:00 [entrez]
AID - S1097-2765(00)80479-7 [pii]
AID - 10.1016/s1097-2765(00)80479-7 [doi]
PST - ppublish
SO  - Mol Cell. 1999 Apr;3(4):513-9. doi: 10.1016/s1097-2765(00)80479-7.