PMID- 10230396
OWN - NLM
STAT- MEDLINE
DCOM- 19990527
LR  - 20190915
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 3
IP  - 4
DP  - 1999 Apr
TI  - Hypermutation in pathogenic bacteria: frequent phase variation in meningococci is
      a phenotypic trait of a specialized mutator biotype.
PG  - 435-45
AB  - Expression of serogroup B meningococcal capsular polysaccharide undergoes
      frequent phase variation involving reversible frameshift mutations within a
      homopolymeric repeat in the siaD gene. A high rate of phase variation is the
      consequence of a biochemical defect in methyl-directed mismatch repair. The
      mutator phenotype is associated to the absence of DNA adenine methyltransferase
      (Dam) activity in all pathogenic isolates and in 50% of commensal strains.
      Analysis of the meningococcal dam gene region revealed that in all Dam- strains a
      gene encoding a putative restriction endonuclease (drg) that cleaves only the
      methylated DNA sequence 5'-GmeATC-3' replaced the dam gene. Insertional
      inactivation of the dam and/or drg genes indicated that high rates of phase
      variation and hypermutator phenotype are caused by absence of a functional dam
      gene.
FAU - Bucci, C
AU  - Bucci C
AD  - Dipartimento di Biologia e Patologia, Cellulare e Molecolare, L. Califano,
      Universita di Napoli Federico II, Centro di Endocrinologia ed Oncologia
      Sperimentale G. Salvatore of the Consiglio Nazionale delle Ricerche, Italy.
FAU - Lavitola, A
AU  - Lavitola A
FAU - Salvatore, P
AU  - Salvatore P
FAU - Del Giudice, L
AU  - Del Giudice L
FAU - Massardo, D R
AU  - Massardo DR
FAU - Bruni, C B
AU  - Bruni CB
FAU - Alifano, P
AU  - Alifano P
LA  - eng
SI  - GENBANK/AF091142
SI  - GENBANK/AF091143
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (RNA, Messenger)
RN  - EC 2.1.1.72 (Dam methyltransferase)
RN  - EC 2.1.1.72 (Site-Specific DNA-Methyltransferase (Adenine-Specific))
RN  - EC 3.1.21.- (DNA Restriction Enzymes)
SB  - IM
MH  - Amino Acid Sequence
MH  - Bacterial Capsules/genetics/immunology
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA Repair/genetics
MH  - DNA Restriction Enzymes/chemistry/*genetics
MH  - Gene Expression Regulation, Bacterial/genetics
MH  - *Genes, Bacterial
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Neisseria meningitidis/*genetics/pathogenicity
MH  - Phenotype
MH  - RNA, Messenger/analysis
MH  - Restriction Mapping
MH  - Sequence Alignment
MH  - Serology
MH  - Site-Specific DNA-Methyltransferase (Adenine-Specific)/chemistry/*genetics
MH  - Transformation, Genetic
EDAT- 1999/05/07 00:00
MHDA- 1999/05/07 00:01
CRDT- 1999/05/07 00:00
PHST- 1999/05/07 00:00 [pubmed]
PHST- 1999/05/07 00:01 [medline]
PHST- 1999/05/07 00:00 [entrez]
AID - S1097-2765(00)80471-2 [pii]
AID - 10.1016/s1097-2765(00)80471-2 [doi]
PST - ppublish
SO  - Mol Cell. 1999 Apr;3(4):435-45. doi: 10.1016/s1097-2765(00)80471-2.