PMID- 10230394
OWN - NLM
STAT- MEDLINE
DCOM- 19990527
LR  - 20190915
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 3
IP  - 4
DP  - 1999 Apr
TI  - Phosphorylation and inactivation of BAD by mitochondria-anchored protein kinase
      A.
PG  - 413-22
AB  - Signaling pathways between cell surface receptors and the BCL-2 family of
      proteins regulate cell death. Survival factors induce the phosphorylation and
      inactivation of BAD, a proapoptotic member. Purification of BAD kinase(s)
      identified membrane-based cAMP-dependent protein kinase (PKA) as a BAD Ser-112
      (S112) site-specific kinase. PKA-specific inhibitors blocked the IL-3-induced
      phosphorylation on S112 of endogenous BAD as well as mitochondria-based BAD S112 
      kinase activity. A blocking peptide that disrupts type II PKA holoenzyme
      association with A-kinase-anchoring proteins (AKAPs) also inhibited BAD
      phosphorylation and eliminated the BAD S112 kinase activity at mitochondria.
      Thus, the anchoring of PKA to mitochondria represents a focused subcellular
      kinase/substrate interaction that inactivates BAD at its target organelle in
      response to a survival factor.
FAU - Harada, H
AU  - Harada H
AD  - Division of Molecular Oncology, Howard Hughes Medical Institute, Washington
      University School of Medicine, St. Louis, Missouri 63110, USA.
FAU - Becknell, B
AU  - Becknell B
FAU - Wilm, M
AU  - Wilm M
FAU - Mann, M
AU  - Mann M
FAU - Huang, L J
AU  - Huang LJ
FAU - Taylor, S S
AU  - Taylor SS
FAU - Scott, J D
AU  - Scott JD
FAU - Korsmeyer, S J
AU  - Korsmeyer SJ
LA  - eng
GR  - 5R01 CA 50239-10/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (Carrier Proteins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Interleukin-3)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (bcl-Associated Death Protein)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinase Type II)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Carrier Proteins/*metabolism
MH  - Cyclic AMP-Dependent Protein Kinase Type II
MH  - Cyclic AMP-Dependent Protein Kinases/*metabolism
MH  - Enzyme Activation
MH  - Enzyme Inhibitors/pharmacology
MH  - Interleukin-3/pharmacology
MH  - Mitochondria/*enzymology
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Signal Transduction
MH  - Tumor Cells, Cultured
MH  - bcl-Associated Death Protein
EDAT- 1999/05/07 00:00
MHDA- 1999/05/07 00:01
CRDT- 1999/05/07 00:00
PHST- 1999/05/07 00:00 [pubmed]
PHST- 1999/05/07 00:01 [medline]
PHST- 1999/05/07 00:00 [entrez]
AID - S1097-2765(00)80469-4 [pii]
AID - 10.1016/s1097-2765(00)80469-4 [doi]
PST - ppublish
SO  - Mol Cell. 1999 Apr;3(4):413-22. doi: 10.1016/s1097-2765(00)80469-4.