PMID- 10229797
OWN - NLM
STAT- MEDLINE
DCOM- 19990614
LR  - 20071115
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 162
IP  - 10
DP  - 1999 May 15
TI  - Cutting edge: identification of the orphan chemokine receptor GPR-9-6 as CCR9,
      the receptor for the chemokine TECK.
PG  - 5671-5
AB  - Thymus-expressed chemokine (TECK) has been reported to chemoattract dendritic
      cells, thymocytes, and activated macrophages. Here, we show that TECK is a
      specific agonist for a human orphan receptor called GPR-9-6. We have determined
      the cDNA sequence of human GPR-9-6 and cloned the corresponding murine cDNA.
      Human and murine GPR-9-6 expression is very high in the thymus and low in lymph
      nodes and spleen. RT-PCR analysis of murine GPR-9-6 expression on murine
      FACS-sorted thymocyte subpopulations showed that this gene is expressed in both
      immature and mature T cells. Additions of human or murine TECK to HEK 293/human
      GPR-9-6 and HEK 293/murine GPR-9-6 transfectants provoked intracytoplasmic
      calcium mobilization. Human TECK also induced the in vitro migration of HEK
      293/human GPR-9-6 cells. These results confirm that GPR-9-6 is a specific
      receptor for TECK. According to the established nomenclature system, we propose
      to rename GPR-9-6 as CC chemokine receptor 9 (CCR9).
FAU - Zaballos, A
AU  - Zaballos A
AD  - Departamento de Inmunologia y Oncologia, Centro Nacional de Biotecnologia,
      Universidad Autonoma de Madrid, Madrid, Spain.
FAU - Gutierrez, J
AU  - Gutierrez J
FAU - Varona, R
AU  - Varona R
FAU - Ardavin, C
AU  - Ardavin C
FAU - Marquez, G
AU  - Marquez G
LA  - eng
SI  - GENBANK/AJ132336
SI  - GENBANK/AJ132337
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (CC chemokine receptor 9)
RN  - 0 (CCL25 protein, human)
RN  - 0 (Ccl25 protein, mouse)
RN  - 0 (Chemokines, CC)
RN  - 0 (Ligands)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR)
RN  - 0 (Receptors, Chemokine)
SB  - AIM
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Calcium Signaling
MH  - Chemokines, CC/*metabolism
MH  - Chemotaxis, Leukocyte
MH  - Cloning, Molecular
MH  - Humans
MH  - Ligands
MH  - Mice
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - RNA, Messenger/isolation & purification
MH  - Receptors, CCR
MH  - Receptors, Chemokine/*genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Thymus Gland/cytology
MH  - Tissue Distribution
EDAT- 1999/05/07 00:00
MHDA- 1999/05/07 00:01
CRDT- 1999/05/07 00:00
PHST- 1999/05/07 00:00 [pubmed]
PHST- 1999/05/07 00:01 [medline]
PHST- 1999/05/07 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1999 May 15;162(10):5671-5.