PMID- 10229796
OWN - NLM
STAT- MEDLINE
DCOM- 19990614
LR  - 20071114
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 162
IP  - 10
DP  - 1999 May 15
TI  - Cutting edge: functional characterization of the effect of the C3H/HeJ defect in 
      mice that lack an Lpsn gene: in vivo evidence for a dominant negative mutation.
PG  - 5666-70
AB  - A point mutation in the Tlr4 gene, which encodes Toll-like receptor 4, has
      recently been proposed to underlie LPS hyporesponsiveness in C3H/HeJ mice (Lpsd).
      The data presented herein demonstrate that F1 progeny from crosses between mice
      that carry a approximately 9-cM deletion of chromosome 4 (including deletion of
      LpsTlr4) and C3H/HeJ mice (i.e., Lps0 x Lpsd F1 mice) exhibit a pattern of LPS
      sensitivity, measured by TNF activity, that is indistinguishable from that
      exhibited by Lpsn x Lpsd F1 progeny and whose average response is "intermediate" 
      to parental responses. Thus, these data provide clear functional support for the 
      hypothesis that the C3H/HeJ defect exerts a dominant negative effect on LPS
      sensitivity; however, expression of a normal Toll-like receptor 4 molecule is
      apparently not required.
FAU - Vogel, S N
AU  - Vogel SN
AD  - Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
      vogel@bob.usuf2.usuhs.mil
FAU - Johnson, D
AU  - Johnson D
FAU - Perera, P Y
AU  - Perera PY
FAU - Medvedev, A
AU  - Medvedev A
FAU - Lariviere, L
AU  - Lariviere L
FAU - Qureshi, S T
AU  - Qureshi ST
FAU - Malo, D
AU  - Malo D
LA  - eng
GR  - AI-18797/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Drosophila Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - AIM
SB  - IM
MH  - Animals
MH  - Chromosome Mapping
MH  - Crosses, Genetic
MH  - *Drosophila Proteins
MH  - Genotype
MH  - Haplotypes
MH  - Lipopolysaccharides/*immunology
MH  - Macrophages/metabolism
MH  - Membrane Glycoproteins/*genetics
MH  - Mice
MH  - Mice, Inbred C3H/*genetics/immunology
MH  - *Mutation, Missense
MH  - NF-kappa B/metabolism
MH  - *Point Mutation
MH  - Receptors, Cell Surface/*genetics
MH  - Sequence Deletion
MH  - Toll-Like Receptor 4
MH  - Toll-Like Receptors
MH  - Tumor Necrosis Factor-alpha/analysis
EDAT- 1999/05/07 00:00
MHDA- 1999/05/07 00:01
CRDT- 1999/05/07 00:00
PHST- 1999/05/07 00:00 [pubmed]
PHST- 1999/05/07 00:01 [medline]
PHST- 1999/05/07 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1999 May 15;162(10):5666-70.