PMID- 10229681
OWN - NLM
STAT- MEDLINE
DCOM- 19990630
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 340 ( Pt 1)
DP  - 1999 May 15
TI  - Human stearoyl-CoA desaturase: alternative transcripts generated from a single
      gene by usage of tandem polyadenylation sites.
PG  - 255-64
AB  - A critical step in the synthesis of unsaturated fatty acids is catalysed by
      stearoyl-CoA desaturase (Scd). To determine the regulation of human Scd, we
      characterized the gene and its transcripts. Screening a human keratinocyte cDNA
      library and analysis of 3'-RACE (rapid amplification of cDNA ends) products from 
      various tissues yielded a 5.2 kb cDNA encoding a 359 amino acid protein with a
      calculated molecular mass of 41.5 kDa. Analysis of 3'-RACE products suggested
      that alternative usage of polyadenylation sites generates two transcripts of 3.9 
      and 5.2 kb, a result consistent with Northern analysis. Southern analysis
      demonstrated the existance of two SCD loci in the human genome. Chromosomal
      mapping localized one locus to chromosome 10, and the second locus to chromosome 
      17. Characterization of genomic clones isolated from chromosome-specific
      libraries revealed that only the locus on chromosome 10 contained introns.
      Sequence analysis of the intron-less locus displayed multiple nucleotide
      insertions and deletions, as well as in-frame stop codons. Reverse
      transcriptase-PCR analysis performed with primers specific to the intron-less
      locus failed to produce a PCR product from brain, liver and skin RNA, indicating 
      that the locus on chromosome 17 is most likely a transcriptionally inactive,
      fully processed pseudogene. These results suggest strongly that there is one
      structural SCD gene in the human genome, and that it generates two transcripts by
      use of alternative polyadenyation sites. Although the primary sequence and
      intron-exon structure of SCD is phylogenetically conserved, divergence between
      rodent and human is seen in the number of SCD genes and in the generation of
      alternative transcripts, suggesting a species-specific component of SCD
      regulation and function.
FAU - Zhang, L
AU  - Zhang L
AD  - Skin Biology T.R.C., Johnson and Johnson, C.P.W.W., Skillman, NJ 08558, USA.
FAU - Ge, L
AU  - Ge L
FAU - Parimoo, S
AU  - Parimoo S
FAU - Stenn, K
AU  - Stenn K
FAU - Prouty, S M
AU  - Prouty SM
LA  - eng
SI  - GENBANK/AF097514
PT  - Journal Article
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (3' Untranslated Regions)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 24937-83-5 (Poly A)
RN  - EC 1.14.19.1 (Stearoyl-CoA Desaturase)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosomes, Human, Pair 10/genetics
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - DNA, Complementary/analysis/genetics
MH  - Genome, Human
MH  - Humans
MH  - Introns/genetics
MH  - Keratinocytes/metabolism
MH  - Molecular Sequence Data
MH  - Poly A/*genetics
MH  - Pseudogenes/genetics
MH  - RNA, Messenger/analysis/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology
MH  - Skin/cytology
MH  - Stearoyl-CoA Desaturase/*genetics
MH  - *Tandem Repeat Sequences
PMC - PMC1220244
EDAT- 1999/05/07 00:00
MHDA- 1999/05/07 00:01
CRDT- 1999/05/07 00:00
PHST- 1999/05/07 00:00 [pubmed]
PHST- 1999/05/07 00:01 [medline]
PHST- 1999/05/07 00:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 May 15;340 ( Pt 1):255-64.