PMID- 10228171
OWN - NLM
STAT- MEDLINE
DCOM- 19990628
LR  - 20181201
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 9
DP  - 1999 May 4
TI  - The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human.
PG  - 2593-609
AB  - Human TAP is an orthologue of the yeast mRNA export factor Mex67p. In mammalian
      cells, TAP has a preferential intranuclear localization, but can also be detected
      at the nuclear pores and shuttles between the nucleus and the cytoplasm. TAP
      directly associates with mRNA in vivo, as it can be UV-crosslinked to poly(A)+
      RNA in HeLa cells. Both the FG-repeat domain of nucleoporin CAN/Nup214 and a
      novel human 15 kDa protein (p15) with homology to NTF2 (a nuclear transport
      factor which associates with RanGDP), directly bind to TAP. When green
      fluorescent protein (GFP)-tagged TAP and p15 are expressed in yeast, they
      localize to the nuclear pores. Strikingly, co-expression of human TAP and p15
      restores growth of the otherwise lethal mex67::HIS3/mtr2::HIS3 double knockout
      strain. Thus, the human TAP-p15 complex can functionally replace the Mex67p-Mtr2p
      complex in yeast and thus performs a conserved role in nuclear mRNA export.
FAU - Katahira, J
AU  - Katahira J
AD  - BZH, Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg,
      Germany.
FAU - Strasser, K
AU  - Strasser K
FAU - Podtelejnikov, A
AU  - Podtelejnikov A
FAU - Mann, M
AU  - Mann M
FAU - Jung, J U
AU  - Jung JU
FAU - Hurt, E
AU  - Hurt E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 2)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Carrier Proteins)
RN  - 0 (MEX67 protein, S cerevisiae)
RN  - 0 (Nuclear Localization Signals)
RN  - 0 (Nuclear Pore Complex Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nucleocytoplasmic Transport Proteins)
RN  - 0 (Nup214 protein, mouse)
RN  - 0 (Nutf2 protein, mouse)
RN  - 0 (Nxt1 protein, mouse)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (TAP1 protein, human)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 2
MH  - ATP-Binding Cassette Transporters
MH  - Amino Acid Sequence
MH  - Biological Transport
MH  - Carrier Proteins/genetics/metabolism
MH  - Cell Nucleus/*metabolism
MH  - Cell-Free System
MH  - Conserved Sequence
MH  - Cytoplasm/metabolism
MH  - Genetic Complementation Test
MH  - Nuclear Envelope/metabolism
MH  - Nuclear Localization Signals
MH  - *Nuclear Pore Complex Proteins
MH  - Nuclear Proteins/genetics/isolation & purification/*metabolism
MH  - *Nucleocytoplasmic Transport Proteins
MH  - Protein Binding
MH  - *Proteins
MH  - RNA, Messenger/*metabolism
MH  - RNA-Binding Proteins/genetics/*metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Homology, Amino Acid
PMC - PMC1171339
EDAT- 1999/05/06 00:00
MHDA- 1999/05/06 00:01
CRDT- 1999/05/06 00:00
PHST- 1999/05/06 00:00 [pubmed]
PHST- 1999/05/06 00:01 [medline]
PHST- 1999/05/06 00:00 [entrez]
AID - 10.1093/emboj/18.9.2593 [doi]
PST - ppublish
SO  - EMBO J. 1999 May 4;18(9):2593-609. doi: 10.1093/emboj/18.9.2593.