PMID- 10228158
OWN - NLM
STAT- MEDLINE
DCOM- 19990628
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 9
DP  - 1999 May 4
TI  - E2F mediates developmental and cell cycle regulation of ORC1 in Drosophila.
PG  - 2435-48
AB  - Throughout the cell cycle of Saccharomyces cerevisiae, the level of origin
      recognition complex (ORC) is constant and ORCs are bound constitutively to
      replication origins. Replication is regulated by the recruitment of additional
      factors such as CDC6. ORC components are widely conserved, and it generally has
      been assumed that they are also stable factors bound to origins throughout the
      cell cycle. In this report, we show that the level of the ORC1 subunit changes
      dramatically throughout Drosophila development. The accumulation of ORC1 is
      regulated by E2F-dependent transcription. In embryos, ORC1 accumulates
      preferentially in proliferating cells. In the eye imaginal disc, ORC1
      accumulation is cell cycle regulated, with high levels in late G1 and S phase. In
      the ovary, the sub-nuclear distribution of ORC1 shifts during a developmentally
      regulated switch from endoreplication of the entire genome to amplification of
      the chorion gene clusters. Furthermore, we find that overexpression of ORC1
      alters the pattern of DNA synthesis in the eye disc and the ovary. Thus,
      replication origin activity appears to be governed in part by the level of ORC1
      in Drosophila.
FAU - Asano, M
AU  - Asano M
AD  - Howard Hughes Medical Institute, Department of Genetics, Box 3657, Duke
      University Medical Center, Durham, NC 27710, USA.
FAU - Wharton, R P
AU  - Wharton RP
LA  - eng
SI  - GENBANK/Y18882
GR  - CA09111-22/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Dp transcription factor, Drosophila)
RN  - 0 (Drosophila Proteins)
RN  - 0 (E2F Transcription Factors)
RN  - 0 (Orc1 protein, Drosophila)
RN  - 0 (Origin Recognition Complex)
RN  - 0 (Retinoblastoma-Binding Protein 1)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - *Carrier Proteins
MH  - Cell Cycle
MH  - *Cell Cycle Proteins
MH  - DNA-Binding Proteins/*biosynthesis/genetics
MH  - Drosophila/*cytology/embryology
MH  - *Drosophila Proteins
MH  - E2F Transcription Factors
MH  - Eye/embryology
MH  - Molecular Sequence Data
MH  - Morphogenesis
MH  - Origin Recognition Complex
MH  - Promoter Regions, Genetic
MH  - Retinoblastoma-Binding Protein 1
MH  - Tissue Distribution
MH  - *Trans-Activators
MH  - Transcription Factors/*metabolism
MH  - Transcription, Genetic
PMC - PMC1171326
EDAT- 1999/05/06 00:00
MHDA- 1999/05/06 00:01
CRDT- 1999/05/06 00:00
PHST- 1999/05/06 00:00 [pubmed]
PHST- 1999/05/06 00:01 [medline]
PHST- 1999/05/06 00:00 [entrez]
AID - 10.1093/emboj/18.9.2435 [doi]
PST - ppublish
SO  - EMBO J. 1999 May 4;18(9):2435-48. doi: 10.1093/emboj/18.9.2435.