PMID- 10228156
OWN - NLM
STAT- MEDLINE
DCOM- 19990628
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 9
DP  - 1999 May 4
TI  - The importin beta/importin 7 heterodimer is a functional nuclear import receptor 
      for histone H1.
PG  - 2411-23
AB  - Import of proteins into the nucleus proceeds through nuclear pore complexes and
      is largely mediated by nuclear transport receptors of the importin beta family
      that use direct RanGTP-binding to regulate the interaction with their cargoes. We
      investigated nuclear import of the linker histone H1 and found that two
      receptors, importin beta (Impbeta) and importin 7 (Imp7, RanBP7), play a critical
      role in this process. Individually, the two import receptors bind H1 weakly, but 
      binding is strong for the Impbeta/Imp7 heterodimer. Consistent with this, import 
      of H1 into nuclei of permeabilized mammalian cells requires exogenous Impbeta
      together with Imp7. Import by the Imp7/Impbeta heterodimer is strictly Ran
      dependent, the Ran-requiring step most likely being the disassembly of the
      cargo-receptor complex following translocation into the nucleus. Disassembly is
      brought about by direct binding of RanGTP to Impbeta and Imp7, whereby the two
      Ran-binding sites act synergistically. However, whereas an Impbeta/RanGTP
      interaction appears essential for H1 import, Ran-binding to Imp7 is dispensable. 
      Thus, Imp7 can function in two modes. Its Ran-binding site is essential when
      operating as an autonomous import receptor, i.e. independently of Impbeta. Within
      the Impbeta/Imp7 heterodimer, however, Imp7 plays a more passive role than
      Impbeta and resembles an import adapter.
FAU - Jakel, S
AU  - Jakel S
AD  - Zentrum fur Molekulare Biologie der Universitat Heidelberg, Im Neuenheimer Feld
      282, D-69120 Heidelberg, Germany.
FAU - Albig, W
AU  - Albig W
FAU - Kutay, U
AU  - Kutay U
FAU - Bischoff, F R
AU  - Bischoff FR
FAU - Schwamborn, K
AU  - Schwamborn K
FAU - Doenecke, D
AU  - Doenecke D
FAU - Gorlich, D
AU  - Gorlich D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Histones)
RN  - 0 (Karyopherins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.6.5.2 (ran GTP-Binding Protein)
SB  - IM
MH  - Allosteric Regulation
MH  - Animals
MH  - Binding Sites
MH  - Biological Transport
MH  - Dimerization
MH  - Guanosine Triphosphate/metabolism
MH  - Histones/*metabolism
MH  - Karyopherins
MH  - Models, Biological
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Point Mutation
MH  - Receptors, Cytoplasmic and Nuclear/*metabolism
MH  - Xenopus
MH  - ran GTP-Binding Protein
PMC - PMC1171324
EDAT- 1999/05/06 00:00
MHDA- 1999/05/06 00:01
CRDT- 1999/05/06 00:00
PHST- 1999/05/06 00:00 [pubmed]
PHST- 1999/05/06 00:01 [medline]
PHST- 1999/05/06 00:00 [entrez]
AID - 10.1093/emboj/18.9.2411 [doi]
PST - ppublish
SO  - EMBO J. 1999 May 4;18(9):2411-23. doi: 10.1093/emboj/18.9.2411.