PMID- 10228148
OWN - NLM
STAT- MEDLINE
DCOM- 19990628
LR  - 20220318
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 9
DP  - 1999 May 4
TI  - Conformation of the Bax C-terminus regulates subcellular location and cell death.
PG  - 2330-41
AB  - Bax, a pro-apoptotic member of the Bcl-2 family, translocates from the cytosol to
      the mitochondria during programmed cell death. We report here that both
      gain-of-function and loss-of-function mutations can be achieved by altering a
      single amino acid in the Bax hydrophobic C-terminus. The properly mutated
      C-terminus of Bax can target a non-relevant protein to the mitochondria, showing 
      that specific conformations of this domain alone allow mitochondrial docking.
      These data along with N-terminus epitope exposure experiments suggest that the C-
      and the N-termini interact and that upon triggering of apoptosis, Bax changes
      conformation, exposing these two domains to insert into the mitochondria and
      regulate the cell death machinery.
FAU - Nechushtan, A
AU  - Nechushtan A
AD  - Biochemistry Section, Surgical Neurology Branch, National Institute of
      Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD
      20892, USA.
FAU - Smith, C L
AU  - Smith CL
FAU - Hsu, Y T
AU  - Hsu YT
FAU - Youle, R J
AU  - Youle RJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (BAX protein, human)
RN  - 0 (BCL2L1 protein, human)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 0 (bcl-X Protein)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Apoptosis
MH  - Biological Transport
MH  - Cell Compartmentation
MH  - Cytoplasm/*metabolism
MH  - Humans
MH  - Mitochondria/*metabolism
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Protein Conformation
MH  - Proto-Oncogene Proteins/chemistry/genetics/*metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Sequence Deletion
MH  - bcl-2-Associated X Protein
MH  - bcl-X Protein
PMC - PMC1171316
EDAT- 1999/05/06 00:00
MHDA- 1999/05/06 00:01
CRDT- 1999/05/06 00:00
PHST- 1999/05/06 00:00 [pubmed]
PHST- 1999/05/06 00:01 [medline]
PHST- 1999/05/06 00:00 [entrez]
AID - 10.1093/emboj/18.9.2330 [doi]
PST - ppublish
SO  - EMBO J. 1999 May 4;18(9):2330-41. doi: 10.1093/emboj/18.9.2330.