PMID- 10228021
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20101118
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 162
IP  - 9
DP  - 1999 May 1
TI  - Response to Listeria monocytogenes in mice lacking MHC class Ia molecules.
PG  - 5429-36
AB  - MHC class Ia-deficient mice (H2 Kb-/- Db-/-) inoculated with the intracellular
      pathogen Listeria monocytogenes (LM) displayed a three- to fourfold expansion of 
      splenic CD8+ T cells 6 days following infection. Culture of these spleen cells in
      vitro gave rise to CTL that recognized LM-infected target cells and were
      restricted by the class Ib molecules, Qa1b and M3. Exposure of target cells to
      heat-killed LM (HKLM) rather than live bacteria did not result in CTL-mediated
      lysis. Target cells pulsed with three LM peptides known to bind M3, f-MIGWII,
      f-MIVTLF, and f-MIVIL, were recognized by effector cells from both B6 and Kb-/-
      Db-/- animals. In vivo analysis showed that B6 and Kb-/- Db-/- mice clear LM from
      the spleen and liver rapidly with similar kinetics, whereas TAP.1-/- mice, which 
      are deficient in class Ia and Ib molecules, clear LM slowly upon infection. To
      establish the in vivo role of CD8+ T cells in Kb-/- Db-/- animals, we showed that
      depletion of such cells from the spleens of immune mice prevented the adoptive
      transfer of protective immunity to syngeneic recipients. Spleen cells from Kb-/- 
      Db-/- mice were also capable of generating responses directed against syngeneic
      as well as allogeneic class Ia molecules in vitro. Thus, class Ia-deficient
      animals have a CD8+ T cell repertoire capable of recognizing both class Ia and
      class Ib molecules and can generate protective immunity to LM.
FAU - Seaman, M S
AU  - Seaman MS
AD  - Immunology Graduate Program, Center for Immunology, University of Texas
      Southwestern Medical Center, Dallas 75235, USA.
FAU - Perarnau, B
AU  - Perarnau B
FAU - Lindahl, K F
AU  - Lindahl KF
FAU - Lemonnier, F A
AU  - Lemonnier FA
FAU - Forman, J
AU  - Forman J
LA  - eng
GR  - P01AI37818/AI/NIAID NIH HHS/United States
GR  - R01AI34930/AI/NIAID NIH HHS/United States
GR  - R01AI37942/AI/NIAID NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (H-2 Antigens)
RN  - 0 (H-2K(K) antigen)
RN  - 0 (H-2Kb protein, mouse)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Q surface antigens)
SB  - AIM
SB  - IM
SB  - X
MH  - Animals
MH  - Antigen Presentation
MH  - Antigens, Bacterial/metabolism
MH  - CD8-Positive T-Lymphocytes/immunology/microbiology
MH  - Epitopes, T-Lymphocyte/genetics/immunology
MH  - H-2 Antigens/*genetics
MH  - Histocompatibility Antigens Class I/genetics
MH  - Listeria monocytogenes/*immunology/pathogenicity
MH  - Listeriosis/genetics/immunology/microbiology/prevention & control
MH  - Liver/microbiology
MH  - Lymphocyte Activation/genetics
MH  - Lymphocyte Count
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Spleen/cytology/immunology/microbiology
MH  - T-Lymphocytes, Cytotoxic/immunology/metabolism/microbiology
MH  - Virulence
EDAT- 1999/05/05 00:00
MHDA- 1999/05/05 00:01
CRDT- 1999/05/05 00:00
PHST- 1999/05/05 00:00 [pubmed]
PHST- 1999/05/05 00:01 [medline]
PHST- 1999/05/05 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1999 May 1;162(9):5429-36.