PMID- 10225960
OWN - NLM
STAT- MEDLINE
DCOM- 19990601
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 145
IP  - 3
DP  - 1999 May 3
TI  - Characterization and expression of the laminin gamma3 chain: a novel,
      non-basement membrane-associated, laminin chain.
PG  - 605-18
AB  - Laminins are heterotrimeric molecules composed of an alpha, a beta, and a gamma
      chain; they have broad functional roles in development and in stabilizing
      epithelial structures. Here, we identified a novel laminin, composed of known
      alpha and beta chains but containing a novel gamma chain, gamma3. We have cloned 
      gene encoding this chain, LAMC3, which maps to chromosome 9 at q31-34. Protein
      and cDNA analyses demonstrate that gamma3 contains all the expected domains of a 
      gamma chain, including two consensus glycosylation sites and a putative
      nidogen-binding site. This suggests that gamma3-containing laminins are likely to
      exist in a stable matrix. Studies of the tissue distribution of gamma3 chain show
      that it is broadly expressed in: skin, heart, lung, and the reproductive tracts. 
      In skin, gamma3 protein is seen within the basement membrane of the
      dermal-epidermal junction at points of nerve penetration. The gamma3 chain is
      also a prominent element of the apical surface of ciliated epithelial cells of:
      lung, oviduct, epididymis, ductus deferens, and seminiferous tubules. The
      distribution of gamma3-containing laminins on the apical surfaces of a variety of
      epithelial tissues is novel and suggests that they are not found within
      ultrastructurally defined basement membranes. It seems likely that these apical
      laminins are important in the morphogenesis and structural stability of the
      ciliated processes of these cells.
FAU - Koch, M
AU  - Koch M
AD  - The Cutaneous Biology Research Center, Massachusetts General Hospital, and the
      Department of Dermatology, Harvard Medical School, Charlestown, Massachusetts
      02129, USA.
FAU - Olson, P F
AU  - Olson PF
FAU - Albus, A
AU  - Albus A
FAU - Jin, W
AU  - Jin W
FAU - Hunter, D D
AU  - Hunter DD
FAU - Brunken, W J
AU  - Brunken WJ
FAU - Burgeson, R E
AU  - Burgeson RE
FAU - Champliaud, M F
AU  - Champliaud MF
LA  - eng
SI  - GENBANK/AF041835
GR  - R01 NS039502/NS/NINDS NIH HHS/United States
GR  - R01 NS039502-03/NS/NINDS NIH HHS/United States
GR  - AR35689/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (DNA, Complementary)
RN  - 0 (Integrins)
RN  - 0 (LAMC3 protein, human)
RN  - 0 (Laminin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Collagen)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibody Specificity
MH  - Basement Membrane
MH  - Blotting, Northern
MH  - Chorionic Villi/chemistry
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 9
MH  - Cilia/chemistry
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Epithelial Cells/chemistry
MH  - Fallopian Tubes/chemistry
MH  - Female
MH  - Humans
MH  - In Situ Hybridization
MH  - Integrins/metabolism
MH  - Laminin/chemistry/*genetics/immunology
MH  - Lung/chemistry
MH  - Male
MH  - Molecular Sequence Data
MH  - Peripheral Nerves/chemistry
MH  - Protein Structure, Tertiary
MH  - RNA, Messenger/analysis
MH  - Receptors, Collagen
MH  - Testis/chemistry
PMC - PMC2185082
EDAT- 1999/05/04 00:00
MHDA- 1999/05/04 00:01
CRDT- 1999/05/04 00:00
PHST- 1999/05/04 00:00 [pubmed]
PHST- 1999/05/04 00:01 [medline]
PHST- 1999/05/04 00:00 [entrez]
AID - 10.1083/jcb.145.3.605 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 May 3;145(3):605-18. doi: 10.1083/jcb.145.3.605.