PMID- 10225435
OWN - NLM
STAT- MEDLINE
DCOM- 19990601
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 449
IP  - 1
DP  - 1999 Apr 16
TI  - Identification, molecular cloning, and characterization of subunit 11 of the
      human 26S proteasome.
PG  - 88-92
AB  - We sequenced five peptides from subunit 11 (S11), a 43 kDa protein of the human
      26S proteasome, and used this information to clone its cDNA. The S11 cDNA encodes
      a 376 amino acid protein with a pI of 5.6 and a molecular mass of 42.9 kDa.
      Translation of S11 RNA in the presence of [35S]methionine produces a radiolabeled
      protein that co-migrates with S11 of the human 26S proteasome on SDS-PAGE.
      Polyclonal antiserum made against recombinant S11 recognizes a protein of the
      same size in extracts of bacteria expressing S11 and in purified 26S proteasomes 
      from human red blood cells or rabbit reticulocytes. The S11 sequence does not
      contain motifs that suggest a biological function. S11 is, however, the human
      homolog of Rpn9, a recently identified subunit of the yeast 26S proteasome.
FAU - Hoffman, L
AU  - Hoffman L
AD  - University of Utah School of Medicine, Department of Biochemistry, Salt Lake City
      84132, USA.
FAU - Gorbea, C
AU  - Gorbea C
FAU - Rechsteiner, M
AU  - Rechsteiner M
LA  - eng
SI  - GENBANK/AF086708
GR  - GM37009/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA, Complementary)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
SB  - IM
MH  - Adenosine Triphosphatases/*genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Rabbits
MH  - Sequence Homology, Amino Acid
EDAT- 1999/05/04 00:00
MHDA- 1999/05/04 00:01
CRDT- 1999/05/04 00:00
PHST- 1999/05/04 00:00 [pubmed]
PHST- 1999/05/04 00:01 [medline]
PHST- 1999/05/04 00:00 [entrez]
AID - S0014-5793(99)00403-2 [pii]
AID - 10.1016/s0014-5793(99)00403-2 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Apr 16;449(1):88-92. doi: 10.1016/s0014-5793(99)00403-2.