PMID- 10224290
OWN - NLM
STAT- MEDLINE
DCOM- 19990615
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 189
IP  - 9
DP  - 1999 May 3
TI  - Role of the scavenger receptor MARCO in alveolar macrophage binding of
      unopsonized environmental particles.
PG  - 1497-506
AB  - Alveolar macrophages (AMs) avidly bind and ingest unopsonized environmental
      particles and bacteria through scavenger-type receptors (SRs). AMs from mice with
      a genetic deletion of the major macrophage SR (types AI and AII; SR-/-) showed no
      decrease in particle binding compared with SR+/+ mice, suggesting that other SRs 
      are involved. To identify these receptors, we generated a monoclonal antibody
      (mAb), PAL-1, that inhibits hamster AM binding of unopsonized particles (TiO2,
      Fe2O3, and latex beads; 66 +/- 5, 77 +/- 2, and 85 +/- 2% inhibition,
      respectively, measured by flow cytometry). This antibody identifies a protein of 
      approximately 70 kD on the AM surface (immunoprecipitation) that is expressed by 
      AMs and other macrophages in situ. A cDNA clone encoding the mAb PAL-1-reactive
      protein isolated by means of COS cell expression was found to be 84 and 77%
      homologous to mouse and human scavenger receptor MARCO mRNA, respectively.
      Transfection of COS cells with MARCO cDNA conferred mAb-inhibitable TiO2 binding.
      Hamster MARCO also mediates AM binding of unopsonized bacteria (67 +/- 5 and 47
      +/- 4% inhibition of Escherichia coli and Staphylococcus aureus binding by mAb
      PAL-1). A polyclonal antibody to human MARCO identified the expected
      approximately 70-kD band on Western blots of lysates of normal bronchoalveolar
      lavage (BAL) cells (>90% AMs) and showed strong immunolabeling of human AMs in
      BAL cytocentrifuge preparations and within lung tissue specimens. In normal mouse
      AMs, the anti-MARCO mAb ED31 also showed immunoreactivity and inhibited binding
      of unopsonized particles (e.g., TiO2 approximately 40%) and bacteria. The novel
      function of binding unopsonized environmental dusts and pathogens suggests an
      important role for MARCO in the lungs' response to inhaled particles.
FAU - Palecanda, A
AU  - Palecanda A
AD  - Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115,
      USA.
FAU - Paulauskis, J
AU  - Paulauskis J
FAU - Al-Mutairi, E
AU  - Al-Mutairi E
FAU - Imrich, A
AU  - Imrich A
FAU - Qin, G
AU  - Qin G
FAU - Suzuki, H
AU  - Suzuki H
FAU - Kodama, T
AU  - Kodama T
FAU - Tryggvason, K
AU  - Tryggvason K
FAU - Koziel, H
AU  - Koziel H
FAU - Kobzik, L
AU  - Kobzik L
LA  - eng
SI  - GENBANK/AF125191
GR  - ES 00002/ES/NIEHS NIH HHS/United States
GR  - ES08129/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (DNA, Complementary)
RN  - 0 (MARCO protein, human)
RN  - 0 (Marco protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Lipoprotein)
RN  - 0 (Receptors, Scavenger)
RN  - 0 (Scarb1 protein, mouse)
RN  - 0 (Scavenger Receptors, Class B)
RN  - 14808-60-7 (Quartz)
RN  - 15FIX9V2JP (titanium dioxide)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Base Sequence
MH  - COS Cells
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA, Complementary
MH  - Escherichia coli/metabolism
MH  - Humans
MH  - Macrophages, Alveolar/*metabolism
MH  - *Membrane Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Precipitin Tests
MH  - Quartz/metabolism
MH  - Receptors, Immunologic/genetics/metabolism/*physiology
MH  - *Receptors, Lipoprotein
MH  - Receptors, Scavenger
MH  - Scavenger Receptors, Class B
MH  - Staphylococcus aureus/metabolism
MH  - Titanium/metabolism
PMC - PMC2193067
EDAT- 1999/05/04 00:00
MHDA- 1999/05/04 00:01
CRDT- 1999/05/04 00:00
PHST- 1999/05/04 00:00 [pubmed]
PHST- 1999/05/04 00:01 [medline]
PHST- 1999/05/04 00:00 [entrez]
AID - 10.1084/jem.189.9.1497 [doi]
PST - ppublish
SO  - J Exp Med. 1999 May 3;189(9):1497-506. doi: 10.1084/jem.189.9.1497.