PMID- 10224279
OWN - NLM
STAT- MEDLINE
DCOM- 19990615
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 189
IP  - 9
DP  - 1999 May 3
TI  - Expression of the c-myc proto-oncogene is essential for HIV-1 infection in
      activated T cells.
PG  - 1391-8
AB  - We previously found that activation of primary CD4+ T cells via both the T cell
      antigen receptor (TCR) and CD28 is required for HIV-1 DNA to be translocated from
      the cytoplasm to the nucleus. Here we report that expression of c-Myc protein in 
      CD4+ T cells is induced only after such costimulation. In addition, cyclosporin A
      not only inhibits nuclear import of HIV-1 DNA but also inhibits expression of
      c-Myc protein. Because of these correlations, we tested whether c-Myc is
      necessary for nuclear import of HIV-1 DNA. Specific c-myc antisense, but not
      sense or non-sense, phosphorothioate oligodeoxynucleotides selectively induced
      the accumulation of two NH2-terminally truncated c-Myc proteins and abolished
      HIV-1 genome entry into host nuclei. Consequently, both virus replication and
      HIV-1-induced apoptotic cell death were inhibited. Synthesis of viral full-length
      DNA was not affected. Specific c-myc antisense oligonucleotide inhibited HIV-1
      infection under conditions that did not affect cell cycle entry or proliferation.
      Thus, c-Myc appears to regulate HIV-1 DNA nuclear import via a mechanism distinct
      from those controlling entry into the cell cycle.
FAU - Sun, Y
AU  - Sun Y
AD  - Regional Primate Research Center, University of Washington, Seattle, Washington
      98195, USA.
FAU - Clark, E A
AU  - Clark EA
LA  - eng
GR  - P51 RR000166/RR/NCRR NIH HHS/United States
GR  - RR00166/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (DNA, Viral)
RN  - 0 (Proto-Oncogene Proteins c-myc)
SB  - IM
SB  - X
MH  - CD4-Positive T-Lymphocytes/metabolism/*virology
MH  - Cells, Cultured
MH  - DNA, Viral/metabolism
MH  - Gene Expression
MH  - HIV-1/genetics/metabolism/*physiology
MH  - Humans
MH  - Lymphocyte Activation
MH  - Proto-Oncogene Proteins c-myc/genetics/*metabolism
PMC - PMC2193066
EDAT- 1999/05/04 00:00
MHDA- 1999/05/04 00:01
CRDT- 1999/05/04 00:00
PHST- 1999/05/04 00:00 [pubmed]
PHST- 1999/05/04 00:01 [medline]
PHST- 1999/05/04 00:00 [entrez]
AID - 10.1084/jem.189.9.1391 [doi]
PST - ppublish
SO  - J Exp Med. 1999 May 3;189(9):1391-8. doi: 10.1084/jem.189.9.1391.