PMID- 10224278
OWN - NLM
STAT- MEDLINE
DCOM- 19990615
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 189
IP  - 9
DP  - 1999 May 3
TI  - Grf40, A novel Grb2 family member, is involved in T cell signaling through
      interaction with SLP-76 and LAT.
PG  - 1383-90
AB  - We molecularly cloned a new Grb2 family member, named Grf40, containing the
      common SH3-SH2-SH3 motif. Expression of Grf40 is predominant in hematopoietic
      cells, particularly T cells. Grf40 binds to the SH2 domain-containing leukocyte
      protein of 76 kD (SLP-76) via its SH3 domain more tightly than Grb2.
      Incidentally, Grf40 binds to linker for activation of T cells (LAT) possibly via 
      its SH2 domain. Overexpression of wild-type Grf40 in Jurkat cells induced a
      significant increase of SLP-76-dependent interleukin (IL)-2 promoter and nuclear 
      factor of activated T cell (NF-AT) activation upon T cell receptor (TCR)
      stimulation, whereas the COOH-terminal SH3-deleted Grf40 mutant lacked any
      recognizable increase in IL-2 promoter activity. Furthermore, the SH2-deleted
      Grf40 mutant led to a marked inhibition of these regulatory activities, the
      effect of which is apparently stronger than that of the SH2-deleted Grb2 mutant. 
      Our data suggest that Grf40 is an adaptor molecule involved in TCR-mediated
      signaling through a more efficient interaction than Grb2 with SLP-76 and LAT.
FAU - Asada, H
AU  - Asada H
AD  - Department of Microbiology and Immunology, Tohoku University School of Medicine, 
      Sendai 980-8575, Japan.
FAU - Ishii, N
AU  - Ishii N
FAU - Sasaki, Y
AU  - Sasaki Y
FAU - Endo, K
AU  - Endo K
FAU - Kasai, H
AU  - Kasai H
FAU - Tanaka, N
AU  - Tanaka N
FAU - Takeshita, T
AU  - Takeshita T
FAU - Tsuchiya, S
AU  - Tsuchiya S
FAU - Konno, T
AU  - Konno T
FAU - Sugamura, K
AU  - Sugamura K
LA  - eng
SI  - GENBANK/AF042380
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (GRAP2 protein, human)
RN  - 0 (GRB2 Adaptor Protein)
RN  - 0 (GRB2 protein, human)
RN  - 0 (Interleukin-2)
RN  - 0 (LAT protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (NFATC Transcription Factors)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (SLP-76 signal Transducing adaptor proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - COS Cells
MH  - Carrier Proteins/*metabolism
MH  - Cell Line
MH  - DNA, Complementary
MH  - DNA-Binding Proteins/metabolism
MH  - GRB2 Adaptor Protein
MH  - HeLa Cells
MH  - Humans
MH  - Interleukin-2/genetics
MH  - Jurkat Cells
MH  - *Membrane Proteins
MH  - Molecular Sequence Data
MH  - NFATC Transcription Factors
MH  - *Nuclear Proteins
MH  - Phosphoproteins/genetics/*metabolism
MH  - Proteins/genetics/*metabolism
MH  - Receptors, Antigen, T-Cell/metabolism
MH  - *Signal Transduction
MH  - T-Lymphocytes/*metabolism
MH  - Transcription Factors/metabolism
MH  - *src Homology Domains
PMC - PMC2193052
EDAT- 1999/05/04 00:00
MHDA- 1999/05/04 00:01
CRDT- 1999/05/04 00:00
PHST- 1999/05/04 00:00 [pubmed]
PHST- 1999/05/04 00:01 [medline]
PHST- 1999/05/04 00:00 [entrez]
AID - 10.1084/jem.189.9.1383 [doi]
PST - ppublish
SO  - J Exp Med. 1999 May 3;189(9):1383-90. doi: 10.1084/jem.189.9.1383.