PMID- 10224116
OWN - NLM
STAT- MEDLINE
DCOM- 19990603
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 19
DP  - 1999 May 7
TI  - Cloning and characterization of the murine Nek3 protein kinase, a novel member of
      the NIMA family of putative cell cycle regulators.
PG  - 13491-7
AB  - We have cloned and characterized murine Nek3 (NIMA-related kinase 3), a novel
      mammalian gene product structurally related to the cell cycle-regulatory kinase
      NIMA of Aspergillus nidulans. By RNase protection, low levels of Nek3 expression 
      could be detected in all organs examined, regardless of proliferative index. In
      contrast to Nek1 and Nek2, Nek3 levels were not particularly elevated in either
      the male or the female germ line. Nek3 levels showed at most marginal variations 
      through the cell cycle, but they were elevated in G0-arrested, quiescent
      fibroblasts. Furthermore, no cell cycle-dependent changes in Nek3 activity could 
      be detected, and no effects upon cell cycle progression could be observed upon
      antibody microinjection or overexpression of either wild-type or catalytically
      inactive Nek3. Finally, Nek3 was found to be a predominantly cytoplasmic enzyme. 
      These data indicate that Nek3 differs from previously characterized Neks with
      regard to all parameters investigated, including organ specificity of expression,
      cell cycle dependence of expression and activity, and subcellular localization.
      Hence, the structural similarity between mammalian Neks may not necessarily be
      indicative of a common function, and it is possible that some members of this
      kinase family may perform functions that are not directly related to cell cycle
      control.
FAU - Tanaka, K
AU  - Tanaka K
AD  - Department of Molecular Biology, Sciences II, University of Geneva 30, Quai
      Ernest-Ansermet, 1211 Geneve 4, Switzerland.
FAU - Nigg, E A
AU  - Nigg EA
LA  - eng
SI  - GENBANK/AF093416
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA, Complementary)
RN  - EC 2.7.11.1 (NEK1 protein, human)
RN  - EC 2.7.11.1 (NEK3 protein, human)
RN  - EC 2.7.11.1 (NIMA-Related Kinase 1)
RN  - EC 2.7.11.1 (NIMA-Related Kinases)
RN  - EC 2.7.11.1 (NIMA-related kinase 6)
RN  - EC 2.7.11.1 (Nek1 protein, mouse)
RN  - EC 2.7.11.1 (Nek3 protein, mouse)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Cycle Proteins/chemistry/genetics/*metabolism
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Female
MH  - Humans
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - NIMA-Related Kinase 1
MH  - NIMA-Related Kinases
MH  - Protein-Serine-Threonine Kinases/chemistry/genetics/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - Subcellular Fractions/enzymology
EDAT- 1999/05/01 00:00
MHDA- 1999/05/01 00:01
CRDT- 1999/05/01 00:00
PHST- 1999/05/01 00:00 [pubmed]
PHST- 1999/05/01 00:01 [medline]
PHST- 1999/05/01 00:00 [entrez]
AID - 10.1074/jbc.274.19.13491 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 May 7;274(19):13491-7. doi: 10.1074/jbc.274.19.13491.