PMID- 10224102
OWN - NLM
STAT- MEDLINE
DCOM- 19990603
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 19
DP  - 1999 May 7
TI  - Molecular cloning of a lipolysis-stimulated remnant receptor expressed in the
      liver.
PG  - 13390-8
AB  - The lipolysis-stimulated receptor (LSR) is a lipoprotein receptor primarily
      expressed in the liver and activated by free fatty acids. Antibodies inhibiting
      LSR functions showed that the receptor is a heterotrimer or tetramer consisting
      of 68-kDa (alpha) and 56-kDa (beta) subunits associated through disulfide
      bridges. Screening of expression libraries with these antibodies led to
      identification of mRNAs derived by alternate splicing from a single gene and
      coding for proteins with molecular masses matching that of LSR alpha and beta.
      Antibodies directed against a synthetic peptide of LSR alpha and beta putative
      ligand binding domains inhibited LSR activity. Western blotting identified two
      liver proteins with the same apparent molecular mass as that of LSR alpha and
      beta. Transient transfections of LSR alpha alone in Chinese hamster ovary cells
      increased oleate-induced binding and uptake of lipoproteins, while cotransfection
      of both LSR alpha and beta increased oleate-induced proteolytic degradation of
      the particles. The ligand specificity of LSR expressed in cotransfected Chinese
      hamster ovary cells closely matched that previously described using fibroblasts
      from subjects lacking the low density lipoprotein receptor. LSR affinity is
      highest for the triglyceride-rich lipoproteins, chylomicrons, and very low
      density lipoprotein. We speculate that LSR is a rate-limiting step for the
      clearance of dietary triglycerides and plays a role in determining their
      partitioning between the liver and peripheral tissues.
FAU - Yen, F T
AU  - Yen FT
AD  - Inserm Unit 391, 35043 Rennes, France.
FAU - Masson, M
AU  - Masson M
FAU - Clossais-Besnard, N
AU  - Clossais-Besnard N
FAU - Andre, P
AU  - Andre P
FAU - Grosset, J M
AU  - Grosset JM
FAU - Bougueleret, L
AU  - Bougueleret L
FAU - Dumas, J B
AU  - Dumas JB
FAU - Guerassimenko, O
AU  - Guerassimenko O
FAU - Bihain, B E
AU  - Bihain BE
LA  - eng
SI  - GENBANK/AF119667
SI  - GENBANK/AF119668
SI  - GENBANK/AF119669
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, LDL)
RN  - 0 (lipolysis-stimulated receptor)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA, Complementary
MH  - Kinetics
MH  - Lipolysis
MH  - Liver/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, LDL/*genetics/metabolism
MH  - Transfection
EDAT- 1999/05/01 00:00
MHDA- 1999/05/01 00:01
CRDT- 1999/05/01 00:00
PHST- 1999/05/01 00:00 [pubmed]
PHST- 1999/05/01 00:01 [medline]
PHST- 1999/05/01 00:00 [entrez]
AID - 10.1074/jbc.274.19.13390 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 May 7;274(19):13390-8. doi: 10.1074/jbc.274.19.13390.