PMID- 10222154
OWN - NLM
STAT- MEDLINE
DCOM- 19990525
LR  - 20051121
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 248
IP  - 2
DP  - 1999 May 1
TI  - cDNA cloning and characterization of PD1: a novel human testicular protein with
      different expressions in various testiculopathies.
PG  - 620-6
AB  - A novel human cDNA sequence has been isolated from human testis cDNA library.
      This sequence, named PD1, reveals an open reading frame encoding a protein of 520
      amino acids. A partial sequence similarity has been found with the RBM gene
      involved in the regulation of human spermatogenesis. Northern blot analysis for
      PD1 mRNA from several human tissues demonstrated two distinct transcripts of 2.7 
      (more abundant) and 4.0 kb and revealed that PD1 is expressed in testis and to a 
      lesser extent also in spleen, thymus, and prostate. Immunohistochemical analysis 
      of human testis showed that this protein is detected in the cytoplasm of Sertoli 
      cells. Antibodies against a rhPD1 fragment were used for Western blot analysis,
      which confirmed the presence of a 60-kDa molecule in crude extract of human
      testicular cells obtained from fine-needle aspiration and showed different
      patterns in various testiculopathies, suggesting a role for such gene in human
      spermatogenesis.
CI  - Copyright 1999 Academic Press.
FAU - Graziotto, R
AU  - Graziotto R
AD  - Institute of Histology and Embryology, Department of Biology, Department of
      Biochemistry, University of Padova, Viale G. Colombo 3, Padua, 35121, Italy.
FAU - Foresta, C
AU  - Foresta C
FAU - Scannapieco, P
AU  - Scannapieco P
FAU - Zeilante, P
AU  - Zeilante P
FAU - Russo, A
AU  - Russo A
FAU - Negro, A
AU  - Negro A
FAU - Salmaso, R
AU  - Salmaso R
FAU - Onisto, M
AU  - Onisto M
LA  - eng
SI  - GENBANK/U28164
PT  - Journal Article
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (DNA, Complementary)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proteins)
RN  - 0 (RBMY1A1 protein, human)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (SPATA2 protein, human)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Blotting, Southern
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - Nuclear Proteins
MH  - Proteins/*genetics/isolation & purification
MH  - RNA-Binding Proteins/genetics
MH  - Seminiferous Tubules/chemistry
MH  - Sequence Homology, Amino Acid
MH  - Sertoli Cells/chemistry
MH  - Sperm Count
MH  - Spermatogenesis
MH  - Testis/*chemistry/*pathology
MH  - Tissue Distribution
EDAT- 1999/05/01 00:00
MHDA- 1999/05/01 00:01
CRDT- 1999/05/01 00:00
PHST- 1999/05/01 00:00 [pubmed]
PHST- 1999/05/01 00:01 [medline]
PHST- 1999/05/01 00:00 [entrez]
AID - S0014-4827(99)94449-X [pii]
AID - 10.1006/excr.1999.4449 [doi]
PST - ppublish
SO  - Exp Cell Res. 1999 May 1;248(2):620-6. doi: 10.1006/excr.1999.4449.