PMID- 10222138
OWN - NLM
STAT- MEDLINE
DCOM- 19990525
LR  - 20181201
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 248
IP  - 2
DP  - 1999 May 1
TI  - Human calumenin localizes to the secretory pathway and is secreted to the medium.
PG  - 473-81
AB  - Calumenin belongs to a family of multiple EF-hand proteins that include
      reticulocalbin, ERC-55, and Cab45. Reticulocalbin and ERC-55 localize to the ER
      due to a C-terminal HDEL retrieval signal. Cab45 contains a HEEF C-terminal
      sequence and is localized to the Golgi apparatus. The murine homologue of
      calumenin is reported to be present in the ER due to a C-terminal HDEF retrieval 
      signal. The human homologue differs from the murine at 7 amino acid positions but
      the HDEF signal is conserved. However, in the cultured human cell lines, HaCaT
      keratinocytes, normal and transformed MRC-5 fibroblasts, as well as in
      transfected COS-1 cells, human calumenin could be demonstrated in the ER as well 
      as in the Golgi complex. Especially in MRC-5 cells, a certain heterogeneity was
      observed, with some of the cells having calumenin localized solely to the ER
      while in other cells calumenin could be demonstrated in the ER as well as in the 
      Golgi complex. Immunoelectron microscopy of placental syncytiotrophoblast cells
      showed that a substantial fraction of calumenin is localized in close association
      with the ER membrane. In addition, the protein may be recovered from the medium
      of cultured cells in an endoglycosidase H-resistant form, suggesting that the
      glycosylated protein has been further modified in the Golgi apparatus and
      secreted to the medium.
CI  - Copyright 1999 Academic Press.
FAU - Vorum, H
AU  - Vorum H
AD  - Department of Medical Biochemistry, University of Aarhus, Ole Worms Alle,
      Building 170, Aarhus C, DK-8000, Denmark.
FAU - Hager, H
AU  - Hager H
FAU - Christensen, B M
AU  - Christensen BM
FAU - Nielsen, S
AU  - Nielsen S
FAU - Honore, B
AU  - Honore B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (CALU protein, human)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Calu protein, mouse)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Antibody Specificity
MH  - Calcium-Binding Proteins/genetics/immunology/*metabolism
MH  - Cell Compartmentation
MH  - Endoplasmic Reticulum
MH  - Fluorescent Antibody Technique, Indirect
MH  - Glycosylation
MH  - Golgi Apparatus
MH  - Humans
MH  - Microscopy, Immunoelectron
MH  - Recombinant Proteins/immunology/metabolism
EDAT- 1999/05/01 00:00
MHDA- 1999/05/01 00:01
CRDT- 1999/05/01 00:00
PHST- 1999/05/01 00:00 [pubmed]
PHST- 1999/05/01 00:01 [medline]
PHST- 1999/05/01 00:00 [entrez]
AID - S0014-4827(99)94431-2 [pii]
AID - 10.1006/excr.1999.4431 [doi]
PST - ppublish
SO  - Exp Cell Res. 1999 May 1;248(2):473-81. doi: 10.1006/excr.1999.4431.