PMID- 10222061
OWN - NLM
STAT- MEDLINE
DCOM- 19990601
LR  - 20171116
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 193
IP  - 2
DP  - 1999 May 1
TI  - Protein kinase C-theta (PKCtheta) distribution analysis in hematopoietic cells:
      proliferating T cells exhibit high proportions of PKCtheta in the particulate
      fraction.
PG  - 185-93
AB  - A comparative analysis of protein kinase C-theta (PKCtheta) protein expression
      was performed in various mouse organs and tissues, freshly isolated populations
      of mouse and human hematopoietic cells, primary leukemias, and established cell
      lines of different histological origins. Results demonstrated a predominant
      expression of PKCtheta in lymphoid tissues and skeletal muscle. Expression levels
      of PKCtheta, as well as PKCalpha, delta, epsilon, zeta, and eta in the thymus,
      were not markedly changed during postnatal development. High levels of expression
      were observed in CD4(+) and CD8(+) single-positive T cells and CD4(+)CD8(+)
      double-positive thymocytes, while B cells were completely devoid of PKCtheta.
      PKCtheta was found also in platelets, but relatively low levels or no detection
      of PKCtheta expression were observed in neutrophils, monocytes, and macrophages. 
      Highly proliferating leukemic T cells of established lines or primary tumors, but
      not freshly isolated resting peripheral blood T cells, exhibited high levels of
      membrane-bound PKCtheta. Increased proportions of PKCtheta in the particulate
      fraction was not restricted to malignant cells but correlated with the extent of 
      proliferation of the T cells. Thus, human peripheral blood T cells that were
      induced to proliferate by exposure to mitogen and IL-2 expressed increased levels
      of PKCtheta in the particulate fraction. Significantly lower proportions of
      membrane-bound PKC were observed for five other isoenzymes expressed in T cells. 
      The occurrence of PKCtheta in T, but not B, cells and its subcellular
      distribution in proliferating cells implicate PKCtheta in cellular mechanisms
      regulating the sustained proliferation of T cells.
CI  - Copyright 1999 Academic Press.
FAU - Meller, N
AU  - Meller N
AD  - Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva,
      84105, Israel.
FAU - Elitzur, Y
AU  - Elitzur Y
FAU - Isakov, N
AU  - Isakov N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Isoenzymes)
RN  - EC 2.7.11.13 (PRKCQ protein, human)
RN  - EC 2.7.11.13 (Prkcq protein, mouse)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.13 (Protein Kinase C-theta)
SB  - IM
MH  - Adult
MH  - Age Factors
MH  - Animals
MH  - Cell Compartmentation
MH  - Hematopoiesis
MH  - Hematopoietic Stem Cells/*enzymology
MH  - Humans
MH  - Isoenzymes/*isolation & purification
MH  - Leukemia, T-Cell/enzymology
MH  - Macrophages
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Models, Biological
MH  - Protein Kinase C/*isolation & purification
MH  - Protein Kinase C-theta
MH  - Subcellular Fractions/enzymology
MH  - T-Lymphocytes/*enzymology
MH  - Thymus Gland/enzymology/growth & development
MH  - Tissue Distribution
MH  - Tumor Cells, Cultured
EDAT- 1999/05/01 00:00
MHDA- 1999/05/01 00:01
CRDT- 1999/05/01 00:00
PHST- 1999/05/01 00:00 [pubmed]
PHST- 1999/05/01 00:01 [medline]
PHST- 1999/05/01 00:00 [entrez]
AID - S0008-8749(99)91478-8 [pii]
AID - 10.1006/cimm.1999.1478 [doi]
PST - ppublish
SO  - Cell Immunol. 1999 May 1;193(2):185-93. doi: 10.1006/cimm.1999.1478.