PMID- 10222050
OWN - NLM
STAT- MEDLINE
DCOM- 19990607
LR  - 20161124
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 365
IP  - 1
DP  - 1999 May 1
TI  - Expression of UDP-glucuronosyltransferases (UGTs) 2B7 and 1A6 in the human brain 
      and identification of 5-hydroxytryptamine as a substrate.
PG  - 156-62
AB  - The extrahepatic expression of UDP-glucuronosyltransferases (UGTs) is important
      in the detoxification of a number of endogenous and exogenous compounds,
      including 5-hydroxytryptamine and morphine. Studies were designed to investigate 
      the extrahepatic expression of human UGTs using RT-PCR techniques and to
      determine the UGTs involved in the glucuronidation of 5-hydroxytryptamine. Human 
      UGT2B7 expression was found in the human liver, kidney, pancreas, and brain,
      while UGT1A6 expression is found in the liver, kidney, and brain. This is the
      first observation of UGTs present in the human central nervous system. Using
      glucuronidation assays, a significant amount of 5-hydroxytryptamine glucuronide
      was found to be catalyzed by UGT1A6. These studies suggest that UGT2B7 may play
      an important role in the overall contribution of morphine analgesia by serving to
      generate the potent morphine-6-O-glucuronide in situ. UGT1A6 could play an
      important role in the glucuronidation of 5-hydroxytryptamine in vivo, therefore
      terminating the actions of the neurotransmitter.
CI  - Copyright 1999 Academic Press.
FAU - King, C D
AU  - King CD
AD  - Department of Pharmacology, College of Medicine, University of Iowa, Iowa City,
      Iowa 52242, USA.
FAU - Rios, G R
AU  - Rios GR
FAU - Assouline, J A
AU  - Assouline JA
FAU - Tephly, T R
AU  - Tephly TR
LA  - eng
GR  - GM26221/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (DNA Primers)
RN  - 0 (Morphine Derivatives)
RN  - 0 (RNA, Messenger)
RN  - 333DO1RDJY (Serotonin)
RN  - 64Y9KYM60R (morphine-6-glucuronide)
RN  - 76I7G6D29C (Morphine)
RN  - EC 2.4.1.- (UDP-glucuronosyltransferase, UGT1A6)
RN  - EC 2.4.1.17 (Glucuronosyltransferase)
SB  - IM
MH  - Adult
MH  - Analgesia
MH  - Brain/*enzymology
MH  - DNA Primers
MH  - Fetus
MH  - Glucuronosyltransferase/genetics/isolation & purification/*metabolism
MH  - Humans
MH  - Inactivation, Metabolic
MH  - Morphine/*metabolism/pharmacology
MH  - Morphine Derivatives/metabolism
MH  - RNA, Messenger/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Serotonin/*metabolism
MH  - Substrate Specificity
MH  - Tissue Distribution
EDAT- 1999/05/01 00:00
MHDA- 1999/05/01 00:01
CRDT- 1999/05/01 00:00
PHST- 1999/05/01 00:00 [pubmed]
PHST- 1999/05/01 00:01 [medline]
PHST- 1999/05/01 00:00 [entrez]
AID - S0003-9861(99)91155-4 [pii]
AID - 10.1006/abbi.1999.1155 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 1999 May 1;365(1):156-62. doi: 10.1006/abbi.1999.1155.